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Yorodumi- PDB-3ks8: Crystal structure of Reston ebolavirus VP35 RNA binding domain in... -
+Open data
-Basic information
Entry | Database: PDB / ID: 3ks8 | ||||||
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Title | Crystal structure of Reston ebolavirus VP35 RNA binding domain in complex with 18bp dsRNA | ||||||
Components |
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Keywords | VIRAL PROTEIN/RNA / ebolavirus / RNA-binding protein / protein-RNA complex / interferon / Reston / Host cytoplasm / Interferon antiviral system evasion / RNA replication / RNA-binding / Transcription / Virion / VIRAL PROTEIN-RNA complex | ||||||
Function / homology | Function and homology information symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF7 activity / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IKBKE activity / virion component / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of TBK1 activity / symbiont-mediated suppression of host toll-like receptor signaling pathway / host cell cytoplasm / RNA binding Similarity search - Function | ||||||
Biological species | Reston ebolavirus | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.401 Å | ||||||
Authors | Kimberlin, C.R. / Bornholdt, Z.A. / Li, S. / Woods, V.L. / Macrae, I.J. / Saphire, E.O. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2009 Title: Ebolavirus VP35 uses a bimodal strategy to bind dsRNA for innate immune suppression. Authors: Kimberlin, C.R. / Bornholdt, Z.A. / Li, S. / Woods, V.L. / Macrae, I.J. / Saphire, E.O. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3ks8.cif.gz | 136.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3ks8.ent.gz | 100.9 KB | Display | PDB format |
PDBx/mmJSON format | 3ks8.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ks/3ks8 ftp://data.pdbj.org/pub/pdb/validation_reports/ks/3ks8 | HTTPS FTP |
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-Related structure data
Related structure data | 3ks4SC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: RNA chain | Mass: 6056.748 Da / Num. of mol.: 1 / Source method: obtained synthetically | ||
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#2: RNA chain | Mass: 5455.203 Da / Num. of mol.: 1 / Source method: obtained synthetically | ||
#3: Protein | Mass: 20569.424 Da / Num. of mol.: 4 Fragment: C-terminal RNA binding domain (UNP residues 160-329) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Reston ebolavirus / Strain: Reston / Gene: REBOVgp2, VP35 / Plasmid: pET46 EK/LIC / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q8JPY0 #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.46 Å3/Da / Density % sol: 49.98 % |
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Crystal grow | Temperature: 295.5 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 365mM ammonium acetate, 100mM trisodium citrate, 18.5% PEG 4000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.5K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SSRL / Beamline: BL11-1 / Wavelength: 0.9795 Å |
Detector | Type: MARMOSAIC 325 mm CCD / Detector: CCD / Date: May 12, 2009 |
Radiation | Monochromator: single crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 2.28→37.1 Å / Num. all: 40821 / Num. obs: 33227 / % possible obs: 81.4 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 4.3 % / Biso Wilson estimate: 42.2 Å2 / Rmerge(I) obs: 0.061 / Net I/σ(I): 23.47 |
Reflection shell | Resolution: 2.28→2.38 Å / Redundancy: 1.43 % / Rmerge(I) obs: 0.254 / Mean I/σ(I) obs: 5.03 / Num. unique all: 1751 / % possible all: 35.3 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB entry 3KS4 Resolution: 2.401→35.117 Å / Occupancy max: 1 / Occupancy min: 0 / SU ML: 0.32 / Cross valid method: THROUGHOUT / σ(F): 1.96 / Phase error: 27.52 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 48.53 Å2 / ksol: 0.369 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 128.04 Å2 / Biso mean: 42.743 Å2 / Biso min: 10.07 Å2
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Refinement step | Cycle: LAST / Resolution: 2.401→35.117 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION
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