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Open data
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Basic information
Entry | Database: PDB / ID: 3kp6 | ||||||
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Title | Staphylococcus epidermidis TcaR in complex with salicylate | ||||||
![]() | Transcriptional regulator TcaR | ||||||
![]() | TRANSCRIPTION REGULATOR / Multiple drug resistance / biofilm / transcription regulation / DNA binding / antibiotics / Transcription | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Chang, Y.M. / Chen, C.K. / Yeh, Y.J. / Wang, A.H. | ||||||
![]() | ![]() Title: Structural study of TcaR and its complexes with multiple antibiotics from Staphylococcus epidermidis. Authors: Chang, Y.M. / Jeng, W.Y. / Ko, T.P. / Yeh, Y.J. / Chen, C.K. / Wang, A.H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 71.6 KB | Display | ![]() |
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PDB format | ![]() | 54.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 471.9 KB | Display | ![]() |
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Full document | ![]() | 486.8 KB | Display | |
Data in XML | ![]() | 14.9 KB | Display | |
Data in CIF | ![]() | 19.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3kp2C ![]() 3kp3C ![]() 3kp4C ![]() 3kp5C ![]() 3kp7SC C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 17378.195 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 35984 / Gene: SERP1949, SE_1937, tcaR / Plasmid: pET 21a / Production host: ![]() ![]() #2: Chemical | ChemComp-SAL / #3: Chemical | ChemComp-CAC / | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.4 Å3/Da / Density % sol: 48.67 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 0.1M Na-Hepes, pH 7.5, 8-14% PEG 4000, 10-13% 2-propanol precipitant, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 210 / Detector: CCD / Date: Jan 10, 2008 / Details: mirrors |
Radiation | Monochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.45→30 Å / Num. all: 12267 / Num. obs: 12192 / % possible obs: 99.4 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 13.9 % / Rmerge(I) obs: 0.073 / Net I/σ(I): 34.3 |
Reflection shell | Resolution: 2.45→2.54 Å / Redundancy: 11 % / Rmerge(I) obs: 0.551 / Mean I/σ(I) obs: 2.1 / Num. unique all: 1126 / % possible all: 77.5 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3KP7 Resolution: 2.45→30 Å / Isotropic thermal model: Isotropic / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 60.82 Å2 | |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.45→30 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.45→2.54 Å
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