[English] 日本語
![](img/lk-miru.gif)
- PDB-3kfv: Crystal structure of the SH3-kinase fragment of tight junction pr... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 3kfv | ||||||
---|---|---|---|---|---|---|---|
Title | Crystal structure of the SH3-kinase fragment of tight junction protein 3 (TJP3) in apo-form | ||||||
![]() | Tight junction protein ZO-3![]() | ||||||
![]() | ![]() ![]() ![]() ![]() ![]() ![]() ![]() | ||||||
Function / homology | ![]() maintenance of blood-brain barrier / bicellular tight junction / ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() ![]() | ||||||
![]() | Tong, Y. / Nedyalkova, L. / Tempel, W. / Zhong, N. / Guan, X. / Arrowsmith, C.H. / Edwards, A.M. / Bountra, C. / Weigelt, J. / Bochkarev, A. ...Tong, Y. / Nedyalkova, L. / Tempel, W. / Zhong, N. / Guan, X. / Arrowsmith, C.H. / Edwards, A.M. / Bountra, C. / Weigelt, J. / Bochkarev, A. / Park, H. / Structural Genomics Consortium (SGC) | ||||||
![]() | ![]() Title: Crystal structure of the SH3-kinase fragment of tight junction protein 3 (TJP3) in apo-form Authors: Tong, Y. / Nedyalkova, L. / Tempel, W. / Zhong, N. / Guan, X. / Arrowsmith, C.H. / Edwards, A.M. / Bountra, C. / Weigelt, J. / Bochkarev, A. / Park, H. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 57.7 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 44 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
---|
-Related structure data
Similar structure data |
---|
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-
Components
#1: Protein | ![]() Mass: 35181.371 Da / Num. of mol.: 1 Fragment: SH3 and Guanylate kinase-like domain residues 489-789 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Plasmid details: pET28a-LIC-CHis (NCBI GI:145307000) Digested with NcoI and BseRI Production host: ![]() ![]() ![]() |
---|---|
#2: Chemical | ChemComp-UNX / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.58 Å3/Da / Density % sol: 52.28 % |
---|---|
Crystal grow![]() | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 5% MPD, 1.4M ammonium sulfate, 0.1M HEPES, 0.01M ADP, pH 7.5, vapor diffusion, hanging drop, temperature 291K |
-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||||||||||||||||||||||||||
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Jul 13, 2009 | ||||||||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength![]() | ||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 2.8→50 Å / Num. obs: 9570 / % possible obs: 99.1 % / Redundancy: 12.9 % / Rmerge(I) obs: 0.155 / Χ2: 1.236 / Net I/σ(I): 5.7 | ||||||||||||||||||||||||||||||||||||
Reflection shell |
|
-Phasing
Phasing![]() | Method: ![]() |
---|
-
Processing
Software |
| ||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure![]() ![]() Details: The programs ARP/WARP (map improvement mode), BUCCANEER, COOT, MOLPROBITY were also used during refinement.
| ||||||||||||||||||||||||||||||||||||
Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 37.639 Å2 / ksol: 0.364 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 93.72 Å2 / Biso mean: 45.35 Å2 / Biso min: 24.89 Å2
| ||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.8→44.327 Å
| ||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||
LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 3
|