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Open data
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Basic information
Entry | Database: PDB / ID: 3kfq | ||||||
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Title | Unreduced cathepsin V in complex with stefin A | ||||||
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![]() | Hydrolase/Hydrolase inhibitor / papain-like cysteine protease / cathepsin / protease-inhibitor complex / Hydrolase / Lysosome / Protease / Thiol protease / Zymogen / Protease inhibitor / Thiol protease inhibitor / Hydrolase-Hydrolase inhibitor complex | ||||||
Function / homology | ![]() cathepsin V / negative regulation of peptidase activity / peptidase inhibitor complex / Formation of the cornified envelope / peptide cross-linking / cornified envelope / RUNX1 regulates transcription of genes involved in differentiation of keratinocytes / Trafficking and processing of endosomal TLR / Assembly of collagen fibrils and other multimeric structures / cysteine-type endopeptidase inhibitor activity ...cathepsin V / negative regulation of peptidase activity / peptidase inhibitor complex / Formation of the cornified envelope / peptide cross-linking / cornified envelope / RUNX1 regulates transcription of genes involved in differentiation of keratinocytes / Trafficking and processing of endosomal TLR / Assembly of collagen fibrils and other multimeric structures / cysteine-type endopeptidase inhibitor activity / Activation of Matrix Metalloproteinases / cysteine-type endopeptidase activator activity involved in apoptotic process / extracellular matrix disassembly / cysteine-type peptidase activity / keratinocyte differentiation / MHC class II antigen presentation / Degradation of the extracellular matrix / lysosomal lumen / proteolysis involved in protein catabolic process / negative regulation of proteolysis / Endosomal/Vacuolar pathway / positive regulation of apoptotic signaling pathway / cell-cell adhesion / antigen processing and presentation of exogenous peptide antigen via MHC class II / protease binding / immune response / cysteine-type endopeptidase activity / serine-type endopeptidase activity / extracellular space / extracellular region / nucleoplasm / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Renko, M. / Turk, D. | ||||||
![]() | ![]() Title: Unreduced cathepsin V in complex with stefin A Authors: Renko, M. / Turk, D. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 149.4 KB | Display | ![]() |
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PDB format | ![]() | 117.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 451.5 KB | Display | ![]() |
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Full document | ![]() | 462.1 KB | Display | |
Data in XML | ![]() | 33.5 KB | Display | |
Data in CIF | ![]() | 49.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1nb3S ![]() 3h6sS ![]() 3a9n S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 24087.053 Da / Num. of mol.: 2 / Mutation: N108Q,N179D Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #2: Protein | Mass: 11020.464 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #3: Water | ChemComp-HOH / | Sequence details | CATHEPSIN V WAS BLOCKED WITH MMTS (METHYL METHANETHIOSULFONATE), LEAVING -S-CH3 ATOMS ON ACTIVE ...CATHEPSIN V WAS BLOCKED WITH MMTS (METHYL METHANETHI | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.63 Å3/Da / Density % sol: 53.19 % / Mosaicity: 0.546 ° |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 8 Details: 0.1M Tris-HCl, 12% PEG3000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MAR CCD 165 mm / Detector: CCD / Date: May 15, 2008 / Details: Collimating and focusing, Pt-coated mirrors |
Radiation | Monochromator: Double Crystal Si111 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.99→22 Å / Num. all: 51300 / Num. obs: 51049 / % possible obs: 99.5 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 / Redundancy: 8.2 % / Rmerge(I) obs: 0.079 / Χ2: 0.88 / Net I/σ(I): 8 |
Reflection shell | Resolution: 2→2.03 Å / Redundancy: 6.8 % / Rmerge(I) obs: 0.43 / Num. unique all: 2302 / Χ2: 0.462 / % possible all: 91.5 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3h6s, 1nb3 Resolution: 1.99→19.49 Å / Cor.coef. Fo:Fc: 0.961 / Cor.coef. Fo:Fc free: 0.941 / WRfactor Rfree: 0.205 / WRfactor Rwork: 0.164 / Occupancy max: 1 / Occupancy min: 0 / FOM work R set: 0.867 / SU R Cruickshank DPI: 0.166 / SU Rfree: 0.151 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.166 / ESU R Free: 0.151 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES: REFINED INDIVIDUALLY. The structure was refined also with MAIN
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 81.22 Å2 / Biso mean: 32.922 Å2 / Biso min: 13.56 Å2
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Refinement step | Cycle: LAST / Resolution: 1.99→19.49 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.995→2.046 Å / Total num. of bins used: 20
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