- PDB-3kdf: X-ray Crystal Structure of the Human Replication Protein A Comple... -
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Basic information
Entry
Database: PDB / ID: 3kdf
Title
X-ray Crystal Structure of the Human Replication Protein A Complex from Wheat Germ Cell Free Expression
Components
Replication protein A 14 kDa subunit
Replication protein A 32 kDa subunit
Keywords
REPLICATION / Wheat germ cell free / protein complex / Center for Eukaryotic Structural Genomics / PSI / replication protein A / Homo sapiens / Protein Structure Initiative / CESG / Acetylation / Alternative splicing / DNA replication / Nucleus / Phosphoprotein / Polymorphism
Function / homology
Function and homology information
protein localization to chromosome / DNA replication factor A complex / regulation of DNA damage checkpoint / Removal of the Flap Intermediate / Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta) / Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha) / Removal of the Flap Intermediate from the C-strand / G-rich strand telomeric DNA binding / HDR through Single Strand Annealing (SSA) / Impaired BRCA2 binding to RAD51 ...protein localization to chromosome / DNA replication factor A complex / regulation of DNA damage checkpoint / Removal of the Flap Intermediate / Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta) / Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha) / Removal of the Flap Intermediate from the C-strand / G-rich strand telomeric DNA binding / HDR through Single Strand Annealing (SSA) / Impaired BRCA2 binding to RAD51 / regulation of double-strand break repair via homologous recombination / telomeric DNA binding / Presynaptic phase of homologous DNA pairing and strand exchange / Activation of the pre-replicative complex / PCNA-Dependent Long Patch Base Excision Repair / Regulation of HSF1-mediated heat shock response / HSF1 activation / mismatch repair / Activation of ATR in response to replication stress / mitotic G1 DNA damage checkpoint signaling / regulation of mitotic cell cycle / telomere maintenance / Translesion synthesis by REV1 / Translesion synthesis by POLK / Translesion synthesis by POLI / Gap-filling DNA repair synthesis and ligation in GG-NER / Fanconi Anemia Pathway / nucleotide-excision repair / Recognition of DNA damage by PCNA-containing replication complex / Termination of translesion DNA synthesis / Translesion Synthesis by POLH / double-strand break repair via homologous recombination / base-excision repair / HDR through Homologous Recombination (HRR) / G2/M DNA damage checkpoint / Dual Incision in GG-NER / PML body / Meiotic recombination / Formation of Incision Complex in GG-NER / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / site of double-strand break / single-stranded DNA binding / regulation of cell population proliferation / Processing of DNA double-strand break ends / protein phosphatase binding / DNA replication / Regulation of TP53 Activity through Phosphorylation / chromosome, telomeric region / damaged DNA binding / nuclear body / DNA repair / ubiquitin protein ligase binding / chromatin / enzyme binding / nucleoplasm / nucleus Similarity search - Function
Replication factor A protein 2 / Replication protein A, C-terminal / Replication protein A C terminal / Replication factor A protein 3 / Replication factor A protein 3 / Replication factor A protein-like / Nucleic acid-binding proteins / OB fold (Dihydrolipoamide Acetyltransferase, E2P) / Winged helix DNA-binding domain superfamily / Winged helix-like DNA-binding domain superfamily ...Replication factor A protein 2 / Replication protein A, C-terminal / Replication protein A C terminal / Replication factor A protein 3 / Replication factor A protein 3 / Replication factor A protein-like / Nucleic acid-binding proteins / OB fold (Dihydrolipoamide Acetyltransferase, E2P) / Winged helix DNA-binding domain superfamily / Winged helix-like DNA-binding domain superfamily / Nucleic acid-binding, OB-fold / Beta Barrel / Mainly Beta Similarity search - Domain/homology
A: Replication protein A 14 kDa subunit D: Replication protein A 32 kDa subunit C: Replication protein A 14 kDa subunit B: Replication protein A 32 kDa subunit hetero molecules
Redundancy: 13.6 % / Av σ(I) over netI: 29.77 / Number: 595468 / Rmerge(I) obs: 0.101 / Χ2: 1.64 / D res high: 1.97 Å / D res low: 50 Å / Num. obs: 43894 / % possible obs: 97.9
Diffraction reflection shell
Highest resolution (Å)
Lowest resolution (Å)
% possible obs (%)
ID
Rmerge(I) obs
Chi squared
Redundancy
5.35
50
99.7
1
0.088
4.87
13.8
4.24
5.35
100
1
0.067
3.302
14.6
3.71
4.24
100
1
0.071
1.886
14.8
3.37
3.71
100
1
0.081
1.844
14.8
3.13
3.37
100
1
0.083
1.874
14.9
2.94
3.13
100
1
0.101
1.591
14.8
2.8
2.94
100
1
0.126
1.304
14.7
2.67
2.8
100
1
0.149
1.302
14.6
2.57
2.67
100
1
0.172
1.281
14.6
2.48
2.57
99.9
1
0.194
1.202
14.5
2.4
2.48
100
1
0.23
1.174
14.4
2.34
2.4
100
1
0.251
1.122
14.3
2.27
2.34
100
1
0.28
1.103
14.1
2.22
2.27
99.9
1
0.306
1.097
13.7
2.17
2.22
99.7
1
0.342
1.091
13.1
2.12
2.17
98.4
1
0.387
1.103
12.5
2.08
2.12
95.9
1
0.443
1.123
11.7
2.04
2.08
92.9
1
0.486
1.121
10.8
2
2.04
88.3
1
0.505
1.143
10
1.97
2
82.7
1
0.566
1.134
8.8
Reflection
Resolution: 1.97→50 Å / Num. obs: 43894 / % possible obs: 97.9 % / Redundancy: 13.6 % / Rmerge(I) obs: 0.101 / Χ2: 1.643 / Net I/σ(I): 12.1