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- PDB-3kcp: Crystal structure of interacting Clostridium thermocellum multimo... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3kcp | ||||||
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Title | Crystal structure of interacting Clostridium thermocellum multimodular components | ||||||
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![]() | STRUCTURAL PROTEIN / Cohesin / Dockerin / X-module / Cellulosome / Carbohydrate metabolism / Cell wall biogenesis/degradation / Cellulose degradation / Glycoprotein / Polysaccharide degradation / Secreted | ||||||
Function / homology | ![]() S-layer / cellulose binding / cellulose catabolic process / polysaccharide catabolic process / hydrolase activity, hydrolyzing O-glycosyl compounds / cell wall organization / carbohydrate binding / extracellular region Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Adams, J.J. / Currie, M.A. / Bayer, E.A. / Jia, Z. / Smith, S.P. | ||||||
![]() | ![]() Title: Insights into Higher-Order Organization of the Cellulosome Revealed by a Dissect-and-Build Approach: Crystal Structure of Interacting Clostridium thermocellum Multimodular Components Authors: Adams, J.J. / Currie, M.A. / Ali, S. / Bayer, E.A. / Jia, Z. / Smith, S.P. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 108.6 KB | Display | ![]() |
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PDB format | ![]() | 81.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 442.1 KB | Display | ![]() |
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Full document | ![]() | 446 KB | Display | |
Data in XML | ![]() | 21.1 KB | Display | |
Data in CIF | ![]() | 30.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Details | The biological assembly is the asymmetric unit |
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Components
#1: Protein | Mass: 34659.938 Da / Num. of mol.: 1 Fragment: Cohesin 9 and Dockerin 1-2 domains: UNP residues 1542-1853 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 27405 / DSM 1237 / Gene: cipA, Cthe_3077 / Production host: ![]() ![]() | ||||
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#2: Protein | Mass: 20566.199 Da / Num. of mol.: 1 / Fragment: UNP residues 27-200 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 27405 / DSM 1237 / Gene: Cthe_1307, SdbA / Production host: ![]() ![]() | ||||
#3: Chemical | #4: Chemical | ChemComp-CL / | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.51 Å3/Da / Density % sol: 50.93 % |
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Crystal grow | Temperature: 294 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 100 mM MES, 200 mM Ammonium sulfate, 30% w/v PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 270 / Detector: CCD / Date: Jul 26, 2005 |
Radiation | Monochromator: Horizontal bent Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9179 Å / Relative weight: 1 |
Reflection | Resolution: 1.94→42.49 Å / Num. all: 41456 / Num. obs: 41456 / % possible obs: 98.67 % / Observed criterion σ(I): 0 / Redundancy: 6.8 % / Rmerge(I) obs: 0.066 / Rsym value: 0.066 / Net I/σ(I): 9.6 |
Reflection shell | Resolution: 1.94→2.05 Å / Redundancy: 5.5 % / Rmerge(I) obs: 0.424 / Mean I/σ(I) obs: 3.2 / Num. unique all: 4056 / Rsym value: 0.424 / % possible all: 98.7 |
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Processing
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Refinement | Method to determine structure: ![]() Stereochemistry target values: MAXIMUM LIKELIHOOD WITH PHASES Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 34.771 Å2
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Refinement step | Cycle: LAST / Resolution: 1.94→42.49 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.94→1.99 Å / Total num. of bins used: 20
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