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Open data
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Basic information
Entry | Database: PDB / ID: 3jw8 | ||||||
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Title | Crystal structure of human mono-glyceride lipase | ||||||
![]() | MGLL protein | ||||||
![]() | HYDROLASE / alpha-beta hydrolase | ||||||
Function / homology | ![]() Arachidonate production from DAG / acylglycerol catabolic process / Acyl chain remodeling of DAG and TAG / acylglycerol lipase / monoacylglycerol catabolic process / triglyceride catabolic process / regulation of endocannabinoid signaling pathway / monoacylglycerol lipase activity / arachidonic acid metabolic process / regulation of sensory perception of pain ...Arachidonate production from DAG / acylglycerol catabolic process / Acyl chain remodeling of DAG and TAG / acylglycerol lipase / monoacylglycerol catabolic process / triglyceride catabolic process / regulation of endocannabinoid signaling pathway / monoacylglycerol lipase activity / arachidonic acid metabolic process / regulation of sensory perception of pain / lysophospholipase activity / Triglyceride catabolism / regulation of signal transduction / lipid metabolic process / fatty acid biosynthetic process / regulation of inflammatory response / inflammatory response / endoplasmic reticulum membrane / protein homodimerization activity / membrane / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Bertrand, T. / Auge, F. / Houtmann, J. / Rak, A. / Vallee, F. / Mikol, V. / Berne, P.F. / Michot, N. / Cheuret, D. / Hoornaert, C. / Mathieu, M. | ||||||
![]() | ![]() Title: Structural basis for human monoglyceride lipase inhibition. Authors: Bertrand, T. / Auge, F. / Houtmann, J. / Rak, A. / Vallee, F. / Mikol, V. / Berne, P.F. / Michot, N. / Cheuret, D. / Hoornaert, C. / Mathieu, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 131.6 KB | Display | ![]() |
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PDB format | ![]() | 108.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 465.7 KB | Display | ![]() |
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Full document | ![]() | 472.5 KB | Display | |
Data in XML | ![]() | 28.5 KB | Display | |
Data in CIF | ![]() | 42.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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3 | ![]()
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4 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 35808.355 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: Q6IBG9, UniProt: Q99685*PLUS, acylglycerol lipase #2: Chemical | #3: Chemical | ChemComp-MRD / ( #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.72 Å3/Da / Density % sol: 54.82 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 6 Details: 50mM MES, 40% MPD, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Jan 28, 2008 | ||||||||||||
Radiation | Monochromator: Si 111 channel / Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||
Radiation wavelength |
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Reflection | Resolution: 2.1→73.9 Å / Num. all: 44315 / Num. obs: 44315 / % possible obs: 97.8 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.6 % / Biso Wilson estimate: 27.56 Å2 / Rsym value: 0.056 / Net I/σ(I): 18.4 | ||||||||||||
Reflection shell | Resolution: 2.1→2.21 Å / Redundancy: 3.7 % / Mean I/σ(I) obs: 6.5 / Rsym value: 0.149 / % possible all: 97.4 |
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Processing
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Refinement | Method to determine structure: ![]()
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Displacement parameters | Biso mean: 27.77 Å2
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Refine analyze | Luzzati coordinate error obs: 0.175 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.1→17.21 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.1→2.15 Å / Total num. of bins used: 20
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