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- PDB-3is2: 2.3 Angstrom Crystal Structure of a Cys71 Sulfenic Acid form of Vivid -
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Open data
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Basic information
Entry | Database: PDB / ID: 3is2 | ||||||
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Title | 2.3 Angstrom Crystal Structure of a Cys71 Sulfenic Acid form of Vivid | ||||||
![]() | (Vivid PAS protein VVD) x 2 | ||||||
![]() | SIGNALING PROTEIN / Photoreceptor / Circadian Clock / Flavin / Sulfenic Acid | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Zoltowski, B.D. / Lamb, J.S. / Pabit, S.A. / Li, L. / Pollack, L. / Crane, B.R. | ||||||
![]() | ![]() Title: Illuminating solution responses of a LOV domain protein with photocoupled small-angle X-ray scattering. Authors: Lamb, J.S. / Zoltowski, B.D. / Pabit, S.A. / Li, L. / Crane, B.R. / Pollack, L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 79.4 KB | Display | ![]() |
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PDB format | ![]() | 63.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 978.4 KB | Display | ![]() |
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Full document | ![]() | 987.6 KB | Display | |
Data in XML | ![]() | 19.6 KB | Display | |
Data in CIF | ![]() | 27.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Details | The crystal structure contains a dimer in the asymmetric unit, however the physiologically relevant photo-induced dimer is currently unknown. |
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Components
#1: Protein | Mass: 17529.004 Da / Num. of mol.: 1 / Fragment: VVD-36 (UNP RESIDUES 37-186) Source method: isolated from a genetically manipulated source Details: CSO at position 71 / Source: (gene. exp.) ![]() ![]() ![]() | ||
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#2: Protein | Mass: 17513.004 Da / Num. of mol.: 1 / Fragment: VVD-36 (UNP RESIDUES 37-186) Source method: isolated from a genetically manipulated source Details: CYS at position 71 / Source: (gene. exp.) ![]() ![]() ![]() | ||
#3: Chemical | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.44 Å3/Da / Density % sol: 49.52 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 5.6 Details: 20 mM imidazole, 26% PEG 4000, 100 mM trisodium citrate, 100 mM NaCl, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 77 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 210 / Detector: CCD / Date: Jun 20, 2007 |
Radiation | Monochromator: Double-bounce downward, offset 25 -110 mm / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.28 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→50 Å / Num. all: 15003 / Num. obs: 14822 / % possible obs: 98.8 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.6 % / Biso Wilson estimate: 33.5 Å2 / Rmerge(I) obs: 0.066 / Net I/σ(I): 24.3 |
Reflection shell | Resolution: 2.3→2.38 Å / Redundancy: 3.5 % / Rmerge(I) obs: 0.2 / Mean I/σ(I) obs: 6 / % possible all: 98.3 |
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Processing
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Refinement | Method to determine structure: ![]()
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Refinement step | Cycle: LAST / Resolution: 2.3→50 Å
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