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- PDB-3hwb: Cation selective pathway of OmpF porin revealed by anomalous diff... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3hwb | ||||||
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Title | Cation selective pathway of OmpF porin revealed by anomalous diffraction | ||||||
![]() | Outer membrane protein F | ||||||
![]() | MEMBRANE PROTEIN / ion transport / porin / integral membrane protein porin / Cell membrane / Cell outer membrane / Membrane / Phage recognition / Transmembrane / Transport | ||||||
Function / homology | ![]() colicin transmembrane transporter activity / monoatomic ion channel complex / porin activity / pore complex / protein homotrimerization / monoatomic ion transmembrane transport / lipopolysaccharide binding / cell outer membrane / disordered domain specific binding / protein transport ...colicin transmembrane transporter activity / monoatomic ion channel complex / porin activity / pore complex / protein homotrimerization / monoatomic ion transmembrane transport / lipopolysaccharide binding / cell outer membrane / disordered domain specific binding / protein transport / monoatomic ion channel activity / lipid binding / identical protein binding / membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Balasundaresan, D. / Raychaudhury, S. / Blachowicz, L. / Roux, B. | ||||||
![]() | ![]() Title: Cation-selective pathway of OmpF porin revealed by anomalous X-ray diffraction. Authors: Dhakshnamoorthy, B. / Raychaudhury, S. / Blachowicz, L. / Roux, B. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 142.7 KB | Display | ![]() |
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PDB format | ![]() | 111.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 734.2 KB | Display | ![]() |
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Full document | ![]() | 738.5 KB | Display | |
Data in XML | ![]() | 25.2 KB | Display | |
Data in CIF | ![]() | 33.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3hw9C ![]() 2omfS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 39365.043 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-RB / #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.94 Å3/Da / Density % sol: 58.21 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6.5 Details: 0.5M NaCl,Na Phosphate buffer pH 6.5, 0.9% BOG, 0.09% octylPOE, sodium azide and 14-18% PEG2000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Jun 8, 2007 |
Radiation | Monochromator: SI(111) Channel / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.8149 Å / Relative weight: 1 |
Reflection | Resolution: 3→50 Å / Num. obs: 17909 / % possible obs: 100 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 4.9 % / Rmerge(I) obs: 0.15 / Net I/σ(I): 9.64 |
Reflection shell | Resolution: 3→3.11 Å / Redundancy: 4.9 % / Rmerge(I) obs: 0.53 / Mean I/σ(I) obs: 2.34 / Num. unique all: 3591 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 2OMF Resolution: 3→46.55 Å / σ(F): 0 / Stereochemistry target values: Engh & Huber Details: Rb peaks selected using SAD phasing and the model predicted by Molecular replacement in CNS
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Refinement step | Cycle: LAST / Resolution: 3→46.55 Å
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