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- PDB-3hva: Crystal structure of FimX GGDEF domain from Pseudomonas aeruginosa -
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Open data
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Basic information
Entry | Database: PDB / ID: 3hva | ||||||
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Title | Crystal structure of FimX GGDEF domain from Pseudomonas aeruginosa | ||||||
![]() | Protein FimX | ||||||
![]() | TRANSFERASE / GGDEF diguanylate cyclase / biofilm / c-di-GMP | ||||||
Function / homology | ![]() phosphorelay signal transduction system / nucleotide binding / regulation of DNA-templated transcription / identical protein binding / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Navarro, M.V.A.S. / De, N. / Bae, N. / Sondermann, H. | ||||||
![]() | ![]() Title: Structural analysis of the GGDEF-EAL domain-containing c-di-GMP receptor FimX. Authors: Navarro, M.V. / De, N. / Bae, N. / Wang, Q. / Sondermann, H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 75.5 KB | Display | ![]() |
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PDB format | ![]() | 55.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 446.4 KB | Display | ![]() |
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Full document | ![]() | 456.1 KB | Display | |
Data in XML | ![]() | 15.9 KB | Display | |
Data in CIF | ![]() | 21.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3hv8C ![]() 3hv9C ![]() 4j40C ![]() 1w25S C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 19132.561 Da / Num. of mol.: 2 / Fragment: GGDEF domain: UNP residues 260-431 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.9 Å3/Da / Density % sol: 35.14 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: PEG MME 5000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 210r / Detector: CCD / Date: Nov 2, 2007 |
Radiation | Monochromator: Si(111) Channel / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9771 Å / Relative weight: 1 |
Reflection twin | Operator: h,-k,-l / Fraction: 0.191 |
Reflection | Resolution: 2→47 Å / Num. all: 18605 / Num. obs: 17415 / % possible obs: 93.6 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 / Redundancy: 3.6 % / Rmerge(I) obs: 0.058 / Net I/σ(I): 18.7 |
Reflection shell | Resolution: 2→2.09 Å / Redundancy: 2.8 % / Rmerge(I) obs: 0.174 / Mean I/σ(I) obs: 6.4 / Num. unique all: 2270 / % possible all: 76.1 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 1W25 Resolution: 2.042→27.118 Å / σ(F): 0.13 / Stereochemistry target values: TWIN_LSQ_F
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 43.249 Å2 / ksol: 0.388 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.042→27.118 Å
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Refine LS restraints |
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LS refinement shell |
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