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- PDB-3hsf: HEAT SHOCK TRANSCRIPTION FACTOR (HSF) -

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Basic information

Entry
Database: PDB / ID: 3hsf
TitleHEAT SHOCK TRANSCRIPTION FACTOR (HSF)
ComponentsHEAT SHOCK TRANSCRIPTION FACTOR
KeywordsTRANSCRIPTION REGULATION
Function / homology
Function and homology information


protein-DNA complex / sequence-specific DNA binding / DNA-binding transcription factor activity / DNA binding / nucleus
Similarity search - Function
HSF-type DNA-binding domain signature. / Heat shock factor (HSF)-type, DNA-binding / HSF-type DNA-binding / heat shock factor / Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain / Arc Repressor Mutant, subunit A / Winged helix DNA-binding domain superfamily / Winged helix-like DNA-binding domain superfamily / Orthogonal Bundle / Mainly Alpha
Similarity search - Domain/homology
Heat shock transcription factor
Similarity search - Component
Biological speciesKluyveromyces lactis (yeast)
MethodSOLUTION NMR
AuthorsDamberger, F.F. / Pelton, J.G. / Liu, C. / Cho, H. / Harrison, C.J. / Nelson, H.C.M. / Wemmer, D.E.
Citation
Journal: J.Mol.Biol. / Year: 1995
Title: Refined solution structure and dynamics of the DNA-binding domain of the heat shock factor from Kluyveromyces lactis.
Authors: Damberger, F.F. / Pelton, J.G. / Liu, C. / Cho, H. / Harrison, C.J. / Nelson, H.C. / Wemmer, D.E.
#1: Journal: Science / Year: 1994
Title: Crystal Structure of the DNA Binding Domain of the Heat Shock Transcription Factor
Authors: Harrison, C.J. / Bohm, A.A. / Nelson, H.C.M.
#2: Journal: Protein Sci. / Year: 1994
Title: Solution Structure of the DNA-Binding Domain of the Heat Shock Transcription Factor Determined by Multidimensional Heteronuclear Magnetic Resonance Spectroscopy
Authors: Damberger, F.F. / Pelton, J.G. / Harrison, C.J. / Nelson, H.C.M. / Wemmer, D.E.
History
DepositionAug 7, 1995Processing site: BNL
SupersessionNov 14, 1995ID: 2HSF
Revision 1.0Nov 14, 1995Provider: repository / Type: Initial release
Revision 1.1Mar 25, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Mar 16, 2022Group: Database references / Derived calculations / Other
Category: database_2 / pdbx_database_status ...database_2 / pdbx_database_status / pdbx_struct_assembly / pdbx_struct_oper_list
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.process_site
Revision 1.4May 22, 2024Group: Data collection / Category: chem_comp_atom / chem_comp_bond

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: HEAT SHOCK TRANSCRIPTION FACTOR


Theoretical massNumber of molelcules
Total (without water)10,9501
Polymers10,9501
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)30 / -
Representative

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Components

#1: Protein HEAT SHOCK TRANSCRIPTION FACTOR / HSF


Mass: 10950.279 Da / Num. of mol.: 1 / Mutation: INS(E89-RHA)
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Kluyveromyces lactis (yeast) / Cell line: BL21(DE3) / Plasmid: PHN212 / Production host: Escherichia coli (E. coli) / References: UniProt: P22121

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR

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Sample preparation

Crystal grow
*PLUS
Method: other

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Processing

Software
NameClassification
X-PLORmodel building
X-PLORrefinement
X-PLORphasing
NMR softwareName: X-PLOR / Developer: BRUNGER / Classification: refinement
NMR ensembleConformers submitted total number: 30

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