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- PDB-3gli: Crystal Structure of the E. coli clamp loader bound to Primer-Tem... -

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Basic information

Entry
Database: PDB / ID: 3gli
TitleCrystal Structure of the E. coli clamp loader bound to Primer-Template DNA and Psi Peptide
Components
  • (DNA polymerase III subunit ...DNA polymerase III holoenzyme) x 4
  • DNA (5'-D(*CP*TP*GP*GP*CP*CP*TP*AP*TP*A)-3')
  • DNA (5'-D(*TP*TP*TP*TP*TP*TP*AP*TP*AP*GP*GP*CP*CP*AP*G)-3')
KeywordsTransferase/DNA / AAA+ ATPase / Clamp Loader / Gamma Complex / Replication / Psi / DNA replication / DNA-directed DNA polymerase / Nucleotidyltransferase / Transferase / ATP-binding / Nucleotide-binding / Transferase-DNA COMPLEX
Function / homology
Function and homology information


DNA polymerase III, clamp loader complex / DNA polymerase III complex / replisome / DNA polymerase processivity factor activity / 3'-5' exonuclease activity / ribonucleoside triphosphate phosphatase activity / response to radiation / DNA-templated DNA replication / DNA replication / DNA-directed DNA polymerase ...DNA polymerase III, clamp loader complex / DNA polymerase III complex / replisome / DNA polymerase processivity factor activity / 3'-5' exonuclease activity / ribonucleoside triphosphate phosphatase activity / response to radiation / DNA-templated DNA replication / DNA replication / DNA-directed DNA polymerase / DNA-directed DNA polymerase activity / ATP hydrolysis activity / DNA binding / ATP binding / identical protein binding
Similarity search - Function
DNA polymerase III, psi subunit / DNA polymerase III, psi subunit, subgroup / DNA polymerase III, psi subunit superfamily / DNA polymerase III psi subunit / Zinc Finger, Delta Prime; domain 3 - #10 / Zinc Finger, Delta Prime; domain 3 / DNA polymerase III, delta prime subunit / DNA polymerase III, delta subunit, C-terminal / : / DNA polymerase III, delta subunit, C terminal ...DNA polymerase III, psi subunit / DNA polymerase III, psi subunit, subgroup / DNA polymerase III, psi subunit superfamily / DNA polymerase III psi subunit / Zinc Finger, Delta Prime; domain 3 - #10 / Zinc Finger, Delta Prime; domain 3 / DNA polymerase III, delta prime subunit / DNA polymerase III, delta subunit, C-terminal / : / DNA polymerase III, delta subunit, C terminal / DNA polymerase III subunit delta', AAA+ ATPase lid domain / DNA polymerase III subunit delta, C-terminal / Processivity clamp loader gamma complex DNA pol III C-term / DNA polymerase III, delta subunit / DNA polymerase III delta, N-terminal / DNA polymerase III, delta subunit / DNA polymerase III, tau subunit, domain V / DNA polymerase III subunit tau, DnaB-binding domain IV / DNA polymerase III, tau subunit, domain V superfamily / DNA polymerase III, subunit gamma/tau, helical lid domain / DNA polymerase III subunits tau domain IV DnaB-binding / DNA polymerase III tau subunit V interacting with alpha / DNA polymerase III, subunit gamma/ tau, N-terminal / DNA polymerase III, gamma subunit, domain III / DNA polymerase III subunits gamma and tau domain III / DNA polymerase III, delta subunit / DNA polymerase III, clamp loader complex, gamma/delta/delta subunit, C-terminal / Ubiquitin-associated (UBA) domain / Helicase, Ruva Protein; domain 3 - #60 / ClpA/B family / Helicase, Ruva Protein; domain 3 / P-loop containing nucleotide triphosphate hydrolases / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / Up-down Bundle / P-loop containing nucleoside triphosphate hydrolase / Rossmann fold / Orthogonal Bundle / 3-Layer(aba) Sandwich / Mainly Alpha / Alpha Beta
Similarity search - Domain/homology
ADENOSINE-5'-DIPHOSPHATE / BERYLLIUM TRIFLUORIDE ION / DNA / DNA (> 10) / DNA polymerase III subunit tau / DNA polymerase III subunit delta / DNA polymerase III subunit delta' / DNA polymerase III subunit psi
Similarity search - Component
Biological speciesEscherichia coli (E. coli)
Escherichia coLI (E. coli)
ESCHERICHIA COLI (E. coli)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.5 Å
AuthorsSimonetta, K.R. / Cantor, A.J. / Kuriyan, J.
CitationJournal: Cell(Cambridge,Mass.) / Year: 2009
Title: The mechanism of ATP-dependent primer-template recognition by a clamp loader complex.
Authors: Simonetta, K.R. / Kazmirski, S.L. / Goedken, E.R. / Cantor, A.J. / Kelch, B.A. / McNally, R. / Seyedin, S.N. / Makino, D.L. / O'Donnell, M. / Kuriyan, J.
History
DepositionMar 12, 2009Deposition site: RCSB / Processing site: RCSB
Revision 1.0May 26, 2009Provider: repository / Type: Initial release
Revision 1.1Jul 13, 2011Group: Version format compliance
Revision 1.2Jan 23, 2013Group: Database references
Revision 1.3Feb 21, 2024Group: Data collection / Database references / Derived calculations
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_struct_conn_angle / struct_conn / struct_ref_seq_dif / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_asym_id / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr2_auth_asym_id / _pdbx_struct_conn_angle.ptnr2_auth_comp_id / _pdbx_struct_conn_angle.ptnr2_auth_seq_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr2_label_atom_id / _pdbx_struct_conn_angle.ptnr2_label_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_ref_seq_dif.details / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: DNA polymerase III subunit delta
B: DNA polymerase III subunit tau
C: DNA polymerase III subunit tau
D: DNA polymerase III subunit tau
E: DNA polymerase III subunit delta'
F: DNA polymerase III subunit delta
G: DNA polymerase III subunit tau
H: DNA polymerase III subunit tau
I: DNA polymerase III subunit tau
J: DNA polymerase III subunit delta'
K: DNA (5'-D(*TP*TP*TP*TP*TP*TP*AP*TP*AP*GP*GP*CP*CP*AP*G)-3')
L: DNA (5'-D(*CP*TP*GP*GP*CP*CP*TP*AP*TP*A)-3')
M: DNA (5'-D(*TP*TP*TP*TP*TP*TP*AP*TP*AP*GP*GP*CP*CP*AP*G)-3')
N: DNA (5'-D(*CP*TP*GP*GP*CP*CP*TP*AP*TP*A)-3')
O: DNA polymerase III subunit psi
P: DNA polymerase III subunit psi
hetero molecules


Theoretical massNumber of molelcules
Total (without water)440,40542
Polymers436,77616
Non-polymers3,62826
Water0
1
A: DNA polymerase III subunit delta
B: DNA polymerase III subunit tau
C: DNA polymerase III subunit tau
D: DNA polymerase III subunit tau
E: DNA polymerase III subunit delta'
K: DNA (5'-D(*TP*TP*TP*TP*TP*TP*AP*TP*AP*GP*GP*CP*CP*AP*G)-3')
L: DNA (5'-D(*CP*TP*GP*GP*CP*CP*TP*AP*TP*A)-3')
O: DNA polymerase III subunit psi
hetero molecules


Theoretical massNumber of molelcules
Total (without water)220,20221
Polymers218,3888
Non-polymers1,81413
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area29090 Å2
ΔGint-153 kcal/mol
Surface area74130 Å2
MethodPISA
2
F: DNA polymerase III subunit delta
G: DNA polymerase III subunit tau
H: DNA polymerase III subunit tau
I: DNA polymerase III subunit tau
J: DNA polymerase III subunit delta'
M: DNA (5'-D(*TP*TP*TP*TP*TP*TP*AP*TP*AP*GP*GP*CP*CP*AP*G)-3')
N: DNA (5'-D(*CP*TP*GP*GP*CP*CP*TP*AP*TP*A)-3')
P: DNA polymerase III subunit psi
hetero molecules


Theoretical massNumber of molelcules
Total (without water)220,20221
Polymers218,3888
Non-polymers1,81413
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area29510 Å2
ΔGint-154 kcal/mol
Surface area75510 Å2
MethodPISA
Unit cell
Length a, b, c (Å)98.690, 217.180, 275.320
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-ID
11
21
12
22
13
23
14
24
15
25
16
26
17
27

NCS domain segments:
Dom-IDComponent-IDEns-IDSelection details
111chain C and (resseq 4:368 or resseq 400:403)
211chain H and (resseq 4:368 or resseq 400:403)
112chain D and (resseq 2:363 or resseq 400:403)
212chain I and (resseq 2:363 or resseq 400:403)
113chain B and (resseq 5:363 or resseq 400:403)
213chain G and (resseq 5:363 or resseq 400:403)
114chain E and (resseq 1:334 or resseq 403)
214chain J and (resseq 1:334 or resseq 403)
115chain A and (resseq 1:333 )
215chain F and (resseq 1:333 )
116chain K or chain L
216chain M or chain N
117chain O and (resseq 2:28)
217chain P and (resseq 2:28)

NCS ensembles :
ID
1
2
3
4
5
6
7

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Components

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DNA polymerase III subunit ... , 4 types, 12 molecules AFBCDGHIEJOP

#1: Protein DNA polymerase III subunit delta / DNA polymerase III holoenzyme


Mass: 38745.574 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Strain: K-12 / Gene: holA, b0640, JW0635 / Production host: Escherichia coli (E. coli) / References: UniProt: P28630, DNA-directed DNA polymerase
#2: Protein
DNA polymerase III subunit tau / DNA polymerase III holoenzyme / DNA polymerase III subunit gamma


Mass: 43955.512 Da / Num. of mol.: 6 / Fragment: UNP residues 1-373
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Strain: K-12 / Gene: dnaX, dnaZ, dnaZX, b0470, JW0459 / Production host: Escherichia coli (E. coli) / References: UniProt: P06710, DNA-directed DNA polymerase
#3: Protein DNA polymerase III subunit delta' / DNA polymerase III holoenzyme


Mass: 36980.484 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coLI (E. coli) / Strain: K-12 / Gene: holB, b1099, JW1085 / Production host: Escherichia coli (E. coli) / References: UniProt: P28631, DNA-directed DNA polymerase
#6: Protein/peptide DNA polymerase III subunit psi / DNA polymerase III holoenzyme


Mass: 3185.556 Da / Num. of mol.: 2 / Fragment: UNP residues 2-28 / Source method: obtained synthetically / Source: (synth.) ESCHERICHIA COLI (E. coli) / References: UniProt: P28632, DNA-directed DNA polymerase

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DNA chain , 2 types, 4 molecules KMLN

#4: DNA chain DNA (5'-D(*TP*TP*TP*TP*TP*TP*AP*TP*AP*GP*GP*CP*CP*AP*G)-3')


Mass: 4589.994 Da / Num. of mol.: 2 / Source method: obtained synthetically
#5: DNA chain DNA (5'-D(*CP*TP*GP*GP*CP*CP*TP*AP*TP*A)-3')


Mass: 3019.991 Da / Num. of mol.: 2 / Source method: obtained synthetically

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Non-polymers , 4 types, 26 molecules

#7: Chemical
ChemComp-ADP / ADENOSINE-5'-DIPHOSPHATE / Adenosine diphosphate


Mass: 427.201 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: C10H15N5O10P2 / Comment: ADP, energy-carrying molecule*YM
#8: Chemical
ChemComp-BEF / BERYLLIUM TRIFLUORIDE ION


Mass: 66.007 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: BeF3
#9: Chemical
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: Mg
#10: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: Zn

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.38 Å3/Da / Density % sol: 63.58 %
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.5
Details: 9% PEG 400, 150 mM MgCl2, 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: ALS / Beamline: 8.2.2 / Wavelength: 1 Å
DetectorType: ADSC QUANTUM 315 / Detector: CCD / Date: Nov 9, 2008
RadiationMonochromator: KOHZU / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 3.5→92.85 Å / Num. all: 75327 / Num. obs: 73670 / % possible obs: 97.8 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1
Reflection shellResolution: 3.5→3.69 Å / % possible all: 97.2

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Processing

Software
NameVersionClassification
HKL-2000data collection
PHASESphasing
PHENIX(phenix.refine)refinement
MOSFLMdata reduction
SCALAdata scaling
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 3.5→73.034 Å / SU ML: 0.46 / σ(F): 1.35 / Phase error: 25.84 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2573 3721 5.06 %
Rwork0.2216 --
obs0.2234 73513 97.41 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 64.003 Å2 / ksol: 0.338 e/Å3
Displacement parameters
Baniso -1Baniso -2Baniso -3
1-25.7914 Å20 Å20 Å2
2---5.5889 Å2-0 Å2
3----20.2026 Å2
Refinement stepCycle: LAST / Resolution: 3.5→73.034 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms27965 974 200 0 29139
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.01329801
X-RAY DIFFRACTIONf_angle_d1.56740698
X-RAY DIFFRACTIONf_dihedral_angle_d18.79411192
X-RAY DIFFRACTIONf_chiral_restr0.0854676
X-RAY DIFFRACTIONf_plane_restr0.0065076
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDTypeRms dev position (Å)
11C2871X-RAY DIFFRACTIONPOSITIONAL
12H2871X-RAY DIFFRACTIONPOSITIONAL0.057
21D2851X-RAY DIFFRACTIONPOSITIONAL
22I2851X-RAY DIFFRACTIONPOSITIONAL0.064
31B2824X-RAY DIFFRACTIONPOSITIONAL
32G2824X-RAY DIFFRACTIONPOSITIONAL0.051
41E2602X-RAY DIFFRACTIONPOSITIONAL
42J2602X-RAY DIFFRACTIONPOSITIONAL0.066
51A2650X-RAY DIFFRACTIONPOSITIONAL
52F2650X-RAY DIFFRACTIONPOSITIONAL0.055
61K487X-RAY DIFFRACTIONPOSITIONAL
62M487X-RAY DIFFRACTIONPOSITIONAL0.062
71O224X-RAY DIFFRACTIONPOSITIONAL
72P224X-RAY DIFFRACTIONPOSITIONAL0.043
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.5-3.54430.34691210.31132521X-RAY DIFFRACTION96
3.5443-3.5910.32941400.29632492X-RAY DIFFRACTION97
3.591-3.64020.30661430.29232538X-RAY DIFFRACTION96
3.6402-3.69220.31271470.27752527X-RAY DIFFRACTION97
3.6922-3.74730.32481420.26982558X-RAY DIFFRACTION98
3.7473-3.80580.31941230.26352561X-RAY DIFFRACTION97
3.8058-3.86820.30661230.26162562X-RAY DIFFRACTION98
3.8682-3.93490.2951410.24672547X-RAY DIFFRACTION97
3.9349-4.00650.30841370.23182550X-RAY DIFFRACTION98
4.0065-4.08350.27421310.24152574X-RAY DIFFRACTION97
4.0835-4.16680.28991320.23792559X-RAY DIFFRACTION97
4.1668-4.25740.24171490.21812587X-RAY DIFFRACTION98
4.2574-4.35650.24331340.21452550X-RAY DIFFRACTION98
4.3565-4.46540.25181320.20222581X-RAY DIFFRACTION98
4.4654-4.58610.23011570.19472576X-RAY DIFFRACTION98
4.5861-4.7210.27481170.20672582X-RAY DIFFRACTION97
4.721-4.87340.23561330.19762600X-RAY DIFFRACTION98
4.8734-5.04750.24451340.20752585X-RAY DIFFRACTION97
5.0475-5.24960.26191150.20492596X-RAY DIFFRACTION97
5.2496-5.48840.24651460.21252581X-RAY DIFFRACTION97
5.4884-5.77770.27911350.20552632X-RAY DIFFRACTION99
5.7777-6.13950.291500.21982612X-RAY DIFFRACTION98
6.1395-6.61320.27341570.21022621X-RAY DIFFRACTION98
6.6132-7.27820.21011420.18512638X-RAY DIFFRACTION99
7.2782-8.33010.19741610.15772641X-RAY DIFFRACTION98
8.3301-10.49010.13791280.12882670X-RAY DIFFRACTION97
10.4901-73.050.23051510.22562751X-RAY DIFFRACTION95
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.89490.71190.36812.5181.87681.69270.2108-0.5692-0.48230.9409-0.71970.87430.3804-0.870501.25720.0272-0.10051.4661-0.20441.4758.054720.259151.512
23.2470.8191-0.30952.81780.52412.599-0.0377-0.08560.6582-0.2717-0.09410.1409-0.9334-0.2712-00.91620.1699-0.26680.7-0.14891.001535.496839.589551.3803
34.4408-1.3145-0.1871.58270.77621.4374-0.04350.04340.1485-0.61630.1438-0.5883-0.56160.496900.6708-0.32060.01870.8492-0.15980.940266.355725.745949.3524
40.8559-0.0180.26853.24770.4792.4695-0.1314-0.1601-0.23890.42810.3152-0.7250.48660.577300.46540.1365-0.12070.9692-0.29311.060371.504-8.017249.4299
51.66741.4371.19072.65371.51032.71590.2012-0.4353-0.15211.01540.017-0.20121.124-0.1642-01.07710.0493-0.05930.7194-0.04220.701348.8326-27.22447.5857
60.92661.00822.84972.6336-0.80222.6725-0.38970.70640.8498-0.0354-0.0027-0.7967-0.65921.2236-01.80420.05790.05341.17260.23151.635511.740822.1262-43.966
73.07470.5429-1.24222.8821-1.39783.6151-0.34290.3070.3698-0.01710.34790.6349-0.3055-0.643401.07870.3070.15680.75160.12040.9708-16.5517-0.2866-45.7219
81.6695-0.51040.58255.4883-1.14022.3668-0.06020.08120.00840.06020.20480.5522-0.0302-0.419100.47520.0370.19920.64840.08420.6082-12.032-32.6174-43.8376
93.8811-1.63711.32341.1263-0.33243.11640.19170.2638-0.39280.0004-0.0874-0.18910.54010.2149-00.6568-0.00050.10090.45580.05350.587618.2645-48.1727-43.0059
103.66021.48362.12421.4005-0.02023.11980.20050.57320.0949-0.37440.0355-0.43590.26890.9964-00.67160.02840.21630.8622-0.04380.714643.4893-33.2307-39.8842
111.58970.3787-0.16012.25640.1891.8437-0.08590.00790.1574-0.24630.0964-0.3212-0.23110.399100.6848-0.07670.00070.7656-0.05950.554648.0611-2.246218.5638
121.75630.13110.37971.6433-0.1821.00140.0274-0.05640.10170.1358-0.01370.1016-0.3347-0.048400.72580.00030.09670.5701-0.03210.421219.0867-24.4006-11.8781
13-0.086-0.554-0.50812.51040.62030.45410.72610.2413-0.0196-0.1097-0.09850.31870.3437-1.4589-00.89570.0116-0.10141.1741-0.18990.88736.69226.220952.5961
141.8491-2.35510.53820.2064-0.94890.06520.0446-0.23650.61130.39030.5744-0.0066-0.99110.6843-01.2159-0.0731-0.00210.85210.08910.745815.8468-10.6791-46.0468
150.8264-0.91520.2380.2507-0.50.6203-0.03491.04770.6476-1.8951-0.15660.5394-1.0838-0.108801.3312-0.16740.30831.2448-0.04780.818357.85615.82775.7366
160.879-0.0512-0.34280.492-0.11440.8246-0.0548-2.01411.37880.79910.28120.5139-0.2411-0.7321-0.00891.0010.08140.33151.0230.10510.68847.8631-31.46960.5376
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain A and resseq 1:211A1 - 211
2X-RAY DIFFRACTION2(chain B and resseq 5:246) or (chain B and (resseq 400:403))B5 - 246
3X-RAY DIFFRACTION2(chain B and resseq 5:246) or (chain B and (resseq 400:403))B400 - 403
4X-RAY DIFFRACTION3(chain C and resseq 4:246) or (chain C and (resseq 400:403))C4 - 246
5X-RAY DIFFRACTION3(chain C and resseq 4:246) or (chain C and (resseq 400:403))C400 - 403
6X-RAY DIFFRACTION4(chain D and resseq 2:246) or (chain D and (resseq 400:403))D2 - 246
7X-RAY DIFFRACTION4(chain D and resseq 2:246) or (chain D and (resseq 400:403))D400 - 403
8X-RAY DIFFRACTION5(chain E and resseq 1:207) or (chain E and resseq 403)E1 - 207
9X-RAY DIFFRACTION5(chain E and resseq 1:207) or (chain E and resseq 403)E403
10X-RAY DIFFRACTION6chain F and resseq 1:211F1 - 211
11X-RAY DIFFRACTION7(chain G and resseq -9:246) or (chain G and (resseq 400:403))G-9 - 246
12X-RAY DIFFRACTION7(chain G and resseq -9:246) or (chain G and (resseq 400:403))G400 - 403
13X-RAY DIFFRACTION8(chain H and resseq 4:246) or (chain H and (resseq 400:403))H4 - 246
14X-RAY DIFFRACTION8(chain H and resseq 4:246) or (chain H and (resseq 400:403))H400 - 403
15X-RAY DIFFRACTION9(chain I and resseq 2:246) or (chain I and (resseq 400:403))I2 - 246
16X-RAY DIFFRACTION9(chain I and resseq 2:246) or (chain I and (resseq 400:403))I400 - 403
17X-RAY DIFFRACTION10(chain J and resseq 1:207) or (chain J and resseq 403)J1 - 207
18X-RAY DIFFRACTION10(chain J and resseq 1:207) or (chain J and resseq 403)J403
19X-RAY DIFFRACTION11(chain A and resseq 212:333) or (chain B and resseq 247:363) or (chain C and resseq 247:368) or (chain D and resseq 247:363) or (chain E and resseq 208:334)A212 - 333
20X-RAY DIFFRACTION11(chain A and resseq 212:333) or (chain B and resseq 247:363) or (chain C and resseq 247:368) or (chain D and resseq 247:363) or (chain E and resseq 208:334)B247 - 363
21X-RAY DIFFRACTION11(chain A and resseq 212:333) or (chain B and resseq 247:363) or (chain C and resseq 247:368) or (chain D and resseq 247:363) or (chain E and resseq 208:334)C247 - 368
22X-RAY DIFFRACTION11(chain A and resseq 212:333) or (chain B and resseq 247:363) or (chain C and resseq 247:368) or (chain D and resseq 247:363) or (chain E and resseq 208:334)D247 - 363
23X-RAY DIFFRACTION11(chain A and resseq 212:333) or (chain B and resseq 247:363) or (chain C and resseq 247:368) or (chain D and resseq 247:363) or (chain E and resseq 208:334)E208 - 334
24X-RAY DIFFRACTION12(chain F and resseq 212:334) or (chain G and resseq 247:363) or (chain H and resseq 247:368) or (chain I and resseq 247:363) or (chain J and resseq 208:334)F212 - 334
25X-RAY DIFFRACTION12(chain F and resseq 212:334) or (chain G and resseq 247:363) or (chain H and resseq 247:368) or (chain I and resseq 247:363) or (chain J and resseq 208:334)G247 - 363
26X-RAY DIFFRACTION12(chain F and resseq 212:334) or (chain G and resseq 247:363) or (chain H and resseq 247:368) or (chain I and resseq 247:363) or (chain J and resseq 208:334)H247 - 368
27X-RAY DIFFRACTION12(chain F and resseq 212:334) or (chain G and resseq 247:363) or (chain H and resseq 247:368) or (chain I and resseq 247:363) or (chain J and resseq 208:334)I247 - 363
28X-RAY DIFFRACTION12(chain F and resseq 212:334) or (chain G and resseq 247:363) or (chain H and resseq 247:368) or (chain I and resseq 247:363) or (chain J and resseq 208:334)J208 - 334
29X-RAY DIFFRACTION13chain K or chain LK2 - 15
30X-RAY DIFFRACTION13chain K or chain LL1 - 10
31X-RAY DIFFRACTION14chain M or chain NM2 - 15
32X-RAY DIFFRACTION14chain M or chain NN1 - 10
33X-RAY DIFFRACTION15chain OO2 - 28
34X-RAY DIFFRACTION16chain PP2 - 28

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