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Open data
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Basic information
Entry | Database: PDB / ID: 3gfi | ||||||
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Title | Crystal structure of ST1710 complexed with its promoter DNA | ||||||
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![]() | TRANSCRIPTION/DNA / Transcription regulator / ST1710 / MarR / DNA-binding / Transcription / Transcription regulation / TRANSCRIPTION-DNA COMPLEX / Structural Genomics / NPPSFA / National Project on Protein Structural and Functional Analyses / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | ![]() response to stress / DNA-binding transcription factor activity / DNA binding / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Kumarevel, T. / Tanaka, T. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
![]() | ![]() Title: ST1710-DNA complex crystal structure reveals the DNA binding mechanism of the MarR family of regulators. Authors: Kumarevel, T. / Tanaka, T. / Umehara, T. / Yokoyama, S. #1: ![]() Title: Crystal structure of the MarR family regulatory protein, ST1710, from Sulfolobus tokodaii strain 7. Authors: Kumarevel, T. / Tanaka, T. / Nishio, M. / Gopinath, S.C. / Takio, K. / Shinkai, A. / Kumar, P.K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 85.4 KB | Display | ![]() |
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PDB format | ![]() | 63.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 455.2 KB | Display | ![]() |
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Full document | ![]() | 465 KB | Display | |
Data in XML | ![]() | 15.8 KB | Display | |
Data in CIF | ![]() | 21.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3gezC ![]() 3gf2C ![]() 3gfjC ![]() 3gflC ![]() 3gfmC C: citing same article ( |
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Similar structure data | |
Other databases |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 16879.564 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: DNA chain | | Mass: 3976.652 Da / Num. of mol.: 1 / Source method: obtained synthetically #3: DNA chain | | Mass: 3041.000 Da / Num. of mol.: 1 / Source method: obtained synthetically #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.54 Å3/Da / Density % sol: 51.61 % | ||||||||||||||||||||||||||||
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 3.8 Details: 30% 2-methyl-2,4-pentanediol (MPD) 0.02M Calcium chloride dehydrate, 0.1M sodium trihydrate, pH 3.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K | ||||||||||||||||||||||||||||
Components of the solutions |
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-Data collection
Diffraction | Mean temperature: 180 K | ||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||
Detector | Type: RIGAKU JUPITER 210 / Detector: CCD / Date: Nov 27, 2008 Details: A fixed exit Si double crystal monochromator followed by a two dimensional focusing mirror which is coated in rhodium | ||||||||||||
Radiation | Monochromator: Fixed exit Si double crystal monochromator, Si (111) Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||
Radiation wavelength |
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Reflection | Resolution: 2.1→40 Å / Num. all: 24231 / Num. obs: 24231 / % possible obs: 99 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 9.6 % / Biso Wilson estimate: 30.3 Å2 / Rsym value: 0.067 | ||||||||||||
Reflection shell | Resolution: 2.1→2.18 Å / Redundancy: 9.8 % / Num. unique all: 2372 / Rsym value: 0.281 / % possible all: 98.6 |
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Processing
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Refinement | Resolution: 2.1→19.75 Å / Rfactor Rfree error: 0.007 / Occupancy max: 1 / Occupancy min: 1 / Data cutoff high absF: 2021016.2 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 54.2158 Å2 / ksol: 0.360857 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 100.15 Å2 / Biso mean: 51.1 Å2 / Biso min: 18.15 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.1→19.75 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.1→2.23 Å / Rfactor Rfree error: 0.017 / Total num. of bins used: 6
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Xplor file |
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