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- PDB-3g7r: Crystal structure of SCO4454, a TetR-family transcriptional regul... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3g7r | ||||||
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Title | Crystal structure of SCO4454, a TetR-family transcriptional regulator from Streptomyces coelicolor | ||||||
![]() | Putative transcriptional regulator | ||||||
![]() | TRANSCRIPTION REGULATOR / TetR / transcriptional regulator / Streptomyces coelicolor / all-helical / Structural Genomics / PSI-2 / Protein Structure Initiative / Midwest Center for Structural Genomics / MCSG / DNA-binding / Transcription / Transcription regulation | ||||||
Function / homology | ![]() transcription cis-regulatory region binding / DNA-binding transcription factor activity / regulation of DNA-templated transcription Similarity search - Function | ||||||
Biological species | Streptomyces coelicolor A3 | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Singer, A.U. / Xu, X. / Chang, C. / Gu, J. / Edwards, A.M. / Joachimiak, A. / Savchenko, A. / Midwest Center for Structural Genomics (MCSG) | ||||||
![]() | ![]() Title: Structure and ligand specificity of SCO4454, a TetR-family transcriptional regulator from Streptomyces coelicolor Authors: Singer, A.U. / Xu, X. / Chang, C. / Edwards, A.M. / Joachimiak, A. / Savchenko, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 195.9 KB | Display | ![]() |
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PDB format | ![]() | 164 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 471.9 KB | Display | ![]() |
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Full document | ![]() | 477.3 KB | Display | |
Data in XML | ![]() | 25.1 KB | Display | |
Data in CIF | ![]() | 38.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
-Protein , 1 types, 2 molecules AB
#1: Protein | Mass: 23954.203 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: A3(2) / M145 / Gene: SCD6.32c, SCO4454 / Plasmid: p15TvLic / Production host: ![]() ![]() |
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-Non-polymers , 6 types, 705 molecules ![](data/chem/img/EDO.gif)
![](data/chem/img/PEG.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/IOD.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/PEG.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/IOD.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | ChemComp-EDO / #3: Chemical | #4: Chemical | #5: Chemical | #6: Chemical | #7: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.41 Å3/Da / Density % sol: 48.96 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop Details: 0.2 M NaI, 20% PEG 3350, cryoprotected in 20% Ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Jun 5, 2006 / Details: mirrors |
Radiation | Monochromator: Si(111) CHANNEL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97937 Å / Relative weight: 1 |
Reflection | Resolution: 1.35→27.39 Å / Num. all: 99985 / Num. obs: 99408 / % possible obs: 99.4 % / Observed criterion σ(I): -3 / Redundancy: 7.1 % / Biso Wilson estimate: 12.5 Å2 / Rmerge(I) obs: 0.079 / Net I/σ(I): 41.361 |
Reflection shell | Resolution: 1.35→1.4 Å / Redundancy: 5.3 % / Rmerge(I) obs: 0.377 / Mean I/σ(I) obs: 4.1 / Num. unique all: 9495 / % possible all: 95.4 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 15.665 Å2
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Refinement step | Cycle: LAST / Resolution: 1.38→27.39 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.38→1.416 Å / Total num. of bins used: 20
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