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Open data
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Basic information
Entry | Database: PDB / ID: 3fzj | ||||||
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Title | TsaR low resolution crystal structure, tetragonal form | ||||||
![]() | LysR type regulator of tsaMBCD | ||||||
![]() | Transcription regulator / LysR-type / transcriptional regulator / TsaR / LTTR / wHTH | ||||||
Function / homology | ![]() transcription cis-regulatory region binding / DNA-binding transcription factor activity Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Monferrer, D. / Tralau, T. / Kertesz, M.A. / Dix, I. / Kikhney, A.G. / Svergun, D.I. / Uson, I. | ||||||
![]() | ![]() Title: Structural studies on the full-length LysR-type regulator TsaR from Comamonas testosteroni T-2 reveal a novel open conformation of the tetrameric LTTR fold Authors: Monferrer, D. / Tralau, T. / Kertesz, M.A. / Dix, I. / Sola, M. / Uson, I. #1: Journal: Acta Crystallogr.,Sect.F / Year: 2008 Title: High crystallizability under air-exclusion conditions of the full-length LysR-type transcriptional regulator TsaR from Comamonas testosteroni T-2 and data-set analysis for a MIRAS structure-solution approach Authors: Monferrer, D. / Tralau, T. / Kertesz, M.A. / Panjikar, S. / Uson, I. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 536.7 KB | Display | ![]() |
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PDB format | ![]() | 446 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 448.4 KB | Display | ![]() |
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Full document | ![]() | 490.8 KB | Display | |
Data in XML | ![]() | 61.1 KB | Display | |
Data in CIF | ![]() | 88.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3fxqSC ![]() 3fxrC ![]() 3fxuC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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3 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 33643.727 Da / Num. of mol.: 10 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Sequence details | THE SEQUENCE OF DATABASE UNIPROTKB/TREMBL P94678 (P94678_COMTE) HAS A SEQUENCING ERROR.THE ...THE SEQUENCE OF DATABASE UNIPROTKB/TREMBL P94678 (P94678_COMTE) HAS A SEQUENCING | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 5.22 Å3/Da / Density % sol: 76.42 % |
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Crystal grow | Temperature: 298 K / pH: 8.5 Details: 2M Ammonium phosphate, 0.1M Tris-HCl, pH 8.5, microbatch under oil, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Jun 27, 2007 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9326 Å / Relative weight: 1 |
Reflection | Resolution: 7→49.57 Å / Num. all: 11860 / Num. obs: 11779 / % possible obs: 99.3 % / Redundancy: 7.73 % / Rmerge(I) obs: 0.1089 / Rsym value: 0.0492 / Net I/σ(I): 17.09 |
Reflection shell | Resolution: 7→7.1 Å / Redundancy: 8.15 % / Rmerge(I) obs: 0.6625 / Mean I/σ(I) obs: 2.74 / Num. unique all: 480 / Rsym value: 0.3896 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 3FXQ Resolution: 7.1→42.34 Å / Occupancy max: 1 / Occupancy min: 0.5 /
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Displacement parameters | Biso max: 46.03 Å2 / Biso mean: 15.571 Å2 / Biso min: 2.58 Å2 | ||||||||||||
Refinement step | Cycle: LAST / Resolution: 7.1→42.34 Å
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