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- PDB-3frl: The 2.25 A crystal structure of LipL32, the major surface antigen... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3frl | ||||||
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Title | The 2.25 A crystal structure of LipL32, the major surface antigen of Leptospira interrogans serovar Copenhageni | ||||||
![]() | LipL32 | ||||||
![]() | MEMBRANE PROTEIN / core jelly-roll fold | ||||||
Function / homology | Surface lipoprotein of Spirochaetales order / Surface lipoprotein of Spirochaetales order / 2,2',2''-NITRILOTRIETHANOL / OXALATE ION / LipL32![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Farah, C.S. / Guzzo, C.R. / Hauk, P. / Ho, P.L. | ||||||
![]() | ![]() Title: Structure and calcium-binding activity of LipL32, the major surface antigen of pathogenic Leptospira sp. Authors: Hauk, P. / Guzzo, C.R. / Roman Ramos, H. / Ho, P.L. / Farah, C.S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 104.5 KB | Display | ![]() |
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PDB format | ![]() | 85.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 27818.822 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: str. Fiocruz L1-130 / Gene: LIC11352, LIC_11352, lipL32 / Plasmid: pAE / Production host: ![]() ![]() #2: Chemical | ChemComp-211 / | #3: Chemical | #4: Chemical | ChemComp-CL / | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4 Å3/Da / Density % sol: 69.24 % |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, sitting drop / pH: 7.7 Details: 1.4 M trisodium citrate, 0.1 M HEPES, 20 mM triethanolamine-HCl, pH 7.7, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Jun 27, 2008 | ||||||||||||
Radiation | Monochromator: Si(111) / Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||
Radiation wavelength |
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Reflection | Resolution: 2.25→40 Å / Num. all: 42419 / Num. obs: 42419 / % possible obs: 99.4 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 10.5 % / Rmerge(I) obs: 0.071 / Net I/σ(I): 22 | ||||||||||||
Reflection shell | Resolution: 2.25→2.33 Å / Redundancy: 5.6 % / Rmerge(I) obs: 0.466 / Mean I/σ(I) obs: 1.9 / % possible all: 95.8 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 40.12 Å2
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Refinement step | Cycle: LAST / Resolution: 2.25→35 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.25→2.308 Å / Total num. of bins used: 20
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