+Open data
-Basic information
Entry | Database: PDB / ID: 3fhu | ||||||
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Title | Crystal structure of type IV b pilin from Salmonella typhi | ||||||
Components | Prepilin | ||||||
Keywords | CELL ADHESION / pilin | ||||||
Function / homology | Type 4 secretion system, PilS, N-terminal / PilS N terminal / TcpA-like pilin / TcpA-like pilin / Pilin-like / 2-Layer Sandwich / membrane / Alpha Beta / Pilus assembly protein PilX Function and homology information | ||||||
Biological species | Salmonella typhi (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MAD / Resolution: 2.1 Å | ||||||
Authors | Balakrishna, A.M. / Swaminathan, K. | ||||||
Citation | Journal: Proteins / Year: 2009 Title: Structural basis of typhoid: Salmonella typhi type IVb pilin (PilS) and cystic fibrosis transmembrane conductance regulator interaction. Authors: Balakrishna, A.M. / Saxena, A.M. / Mok, H.Y. / Swaminathan, K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3fhu.cif.gz | 64 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3fhu.ent.gz | 51.6 KB | Display | PDB format |
PDBx/mmJSON format | 3fhu.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 3fhu_validation.pdf.gz | 437.5 KB | Display | wwPDB validaton report |
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Full document | 3fhu_full_validation.pdf.gz | 445.7 KB | Display | |
Data in XML | 3fhu_validation.xml.gz | 16 KB | Display | |
Data in CIF | 3fhu_validation.cif.gz | 22.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fh/3fhu ftp://data.pdbj.org/pub/pdb/validation_reports/fh/3fhu | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 15842.432 Da / Num. of mol.: 2 / Fragment: residues 26-181 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Salmonella typhi (bacteria) / Gene: pilS, STY4547, t4247 / Plasmid: Pet32a / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 DE3 / References: UniProt: Q8Z1L1 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.23 Å3/Da / Density % sol: 44.95 % |
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Crystal grow | Temperature: 294 K / Method: vapor diffusion, sitting drop Details: 4M Sodium Formate, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X12C / Wavelength: 0.97939, 0.97978, 0.95 | ||||||||||||
Detector | Type: Brandeis B4 / Detector: CCD / Date: Oct 9, 2004 | ||||||||||||
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||
Radiation wavelength |
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Reflection | Resolution: 2.1→8 Å / Num. all: 22854 / Num. obs: 21898 / % possible obs: 95.8 % / Observed criterion σ(F): 3 / Observed criterion σ(I): 1 / Redundancy: 4.3 % / Rsym value: 0.029 / Net I/σ(I): 26.5 | ||||||||||||
Reflection shell | Resolution: 2.1→2.18 Å / Redundancy: 4.3 % / Mean I/σ(I) obs: 7.39 / Num. unique all: 23297 / Rsym value: 0.15 |
-Processing
Software |
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Refinement | Method to determine structure: MAD / Resolution: 2.1→8 Å / σ(F): 3 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.1→8 Å
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LS refinement shell | Resolution: 2.1→2.18 Å
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