- PDB-3fgs: Crystal structure of G65R/K206E double mutant of the N-lobe human... -
+
データを開く
IDまたはキーワード:
読み込み中...
-
基本情報
登録情報
データベース: PDB / ID: 3fgs
タイトル
Crystal structure of G65R/K206E double mutant of the N-lobe human transferrin
要素
Serotransferrin
キーワード
METAL TRANSPORT / Human transferrin (トランスフェリン) / Iron binding protein / Dilysine pair / Disease mutation / Glycoprotein (糖タンパク質) / Ion transport / Iron (鉄) / Iron transport / Metal-binding / Methylation (メチル化) / Phosphoprotein / Polymorphism / Secreted (分泌) / Transport
機能・相同性
機能・相同性情報
iron chaperone activity / Transferrin endocytosis and recycling / transferrin receptor binding / basal part of cell / positive regulation of cell motility / endocytic vesicle / positive regulation of bone resorption / クラスリン / positive regulation of phosphorylation / ERK1 and ERK2 cascade ...iron chaperone activity / Transferrin endocytosis and recycling / transferrin receptor binding / basal part of cell / positive regulation of cell motility / endocytic vesicle / positive regulation of bone resorption / クラスリン / positive regulation of phosphorylation / ERK1 and ERK2 cascade / ferric iron binding / basal plasma membrane / osteoclast differentiation / actin filament organization / Iron uptake and transport / Post-translational protein phosphorylation / ferrous iron binding / clathrin-coated endocytic vesicle membrane / regulation of protein stability / regulation of iron ion transport / HFE-transferrin receptor complex / recycling endosome / cellular response to iron ion / positive regulation of receptor-mediated endocytosis / Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) / Cargo recognition for clathrin-mediated endocytosis / late endosome / Platelet degranulation / Clathrin-mediated endocytosis / iron ion transport / cytoplasmic vesicle / antibacterial humoral response / secretory granule lumen / intracellular iron ion homeostasis / vesicle / blood microparticle / エンドソーム / endosome membrane / apical plasma membrane / 小胞体 / perinuclear region of cytoplasm / positive regulation of DNA-templated transcription / 細胞膜 / extracellular space / extracellular exosome / extracellular region / 細胞膜 類似検索 - 分子機能
温度: 293 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 7.7 詳細: 200 mM potassium acetate buffer (pH 7.7) containing 10 mM KCl and 18% polyethylene glycol 3350. Concentration of the mutant was 17.5 mg/mL. Crystals appeared in approximately a week following ...詳細: 200 mM potassium acetate buffer (pH 7.7) containing 10 mM KCl and 18% polyethylene glycol 3350. Concentration of the mutant was 17.5 mg/mL. Crystals appeared in approximately a week following micro-seeding with wild-type N-lobe, VAPOR DIFFUSION, HANGING DROP, temperature 293K
-
データ収集
回折
平均測定温度: 100 K
放射光源
由来: 回転陽極 / タイプ: RIGAKU RU300 / 波長: 1.5418 Å
検出器
タイプ: MAR scanner 345 mm plate / 検出器: IMAGE PLATE / 日付: 2001年8月7日 / 詳細: Xenocs FOX-2D Multilayer Mirrors
放射
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 1.5418 Å / 相対比: 1
反射
解像度: 1.8→30 Å / Num. obs: 29637 / % possible obs: 93.3 % / Rmerge(I) obs: 0.049 / Χ2: 1.511
反射 シェル
解像度 (Å)
Rmerge(I) obs
Num. unique all
Χ2
% possible all
1.8-1.86
0.113
2701
1.949
87.2
1.86-1.94
0.09
2819
1.927
90.3
1.94-2.03
0.077
2863
1.753
91.9
2.03-2.13
0.074
2908
1.84
92.7
2.13-2.27
0.062
2976
1.713
94.4
2.27-2.44
0.055
2999
1.497
95.2
2.44-2.69
0.052
3030
1.415
95.9
2.69-3.08
0.047
3089
1.318
96.7
3.08-3.88
0.043
3134
1.112
97
3.88-30
0.042
3118
1.097
91.4
-
位相決定
位相決定
手法: 分子置換
Phasing MR
Method rotation: fast direct / Method translation: &STRIP%trans_method