[English] 日本語
![](img/lk-miru.gif)
- PDB-3fb5: KcsA potassium channel in the partially open state with 14.5 A op... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 3fb5 | ||||||
---|---|---|---|---|---|---|---|
Title | KcsA potassium channel in the partially open state with 14.5 A opening at T112 | ||||||
![]() |
| ||||||
![]() | membrane protein/metal transport / kcsa / open / inactivation / potassium channel / Cell membrane / Ion transport / Ionic channel / Membrane / Transmembrane / Transport / Voltage-gated channel / membrane protein-metal transport COMPLEX | ||||||
Function / homology | ![]() delayed rectifier potassium channel activity / voltage-gated potassium channel complex / identical protein binding Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Cuello, L.G. / Jogini, V. / Cortes, D.M. / Perozo, E. | ||||||
![]() | ![]() Title: KcsA potassium channel in the partially open state with 14.5 A opening at T112 Authors: Cuello, L.G. / Jogini, V. / Cortes, D.M. / Perozo, E. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 112.1 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 86.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 438.4 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 454.9 KB | Display | |
Data in XML | ![]() | 21.9 KB | Display | |
Data in CIF | ![]() | 29.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1k4cS S: Starting model for refinement |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| |||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| |||||||||||||||||||||
2 |
| |||||||||||||||||||||
Unit cell |
| |||||||||||||||||||||
Components on special symmetry positions |
|
-
Components
#1: Antibody | Mass: 23411.242 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() | ||
---|---|---|---|
#2: Antibody | Mass: 23435.738 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() | ||
#3: Protein | Mass: 10927.571 Da / Num. of mol.: 1 / Fragment: UNP residues 21-124 / Mutation: H25Q,L90C,R117Q,E120Q,R121Q,R122Q,H124Q Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() | ||
#4: Chemical | ChemComp-K / #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 4.01 Å3/Da / Density % sol: 69.34 % |
---|---|
Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop Details: 20-25% PEG400, 50mM magnesium acetate, 50mM sodium acetate pH 5.0-6.0, pH 5-6, VAPOR DIFFUSION, SITTING DROP, temperature 298K PH range: 5-6 |
-Data collection
Diffraction source | Source: ![]() ![]() ![]() |
---|---|
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Nov 6, 2006 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→40 Å / Num. all: 22718 / Num. obs: 19959 / % possible obs: 87.9 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 |
-
Processing
Software |
| ||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: PDB entry 1K4C Resolution: 2.8→40 Å / σ(F): 2
| ||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.8→40 Å
| ||||||||||||||||||
Refine LS restraints |
|