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Open data
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Basic information
Entry | Database: PDB / ID: 6w0f | ||||||
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Title | Closed-gate KcsA soaked in 0mM KCl/5mM BaCl2 | ||||||
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![]() | MEMBRANE PROTEIN / Ion channel | ||||||
Function / homology | ![]() delayed rectifier potassium channel activity / voltage-gated potassium channel complex / identical protein binding Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Rohaim, A. / Gong, L. / Li, J. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Open and Closed Structures of a Barium-Blocked Potassium Channel. Authors: Rohaim, A. / Gong, L. / Li, J. / Rui, H. / Blachowicz, L. / Roux, B. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 115.2 KB | Display | ![]() |
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PDB format | ![]() | 86.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 6w0aC ![]() 6w0bC ![]() 6w0cC ![]() 6w0dC ![]() 6w0eC ![]() 6w0gC ![]() 6w0hC ![]() 6w0iC ![]() 6w0jC ![]() 1k4cS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Antibody | Mass: 23411.242 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Antibody | Mass: 23435.738 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
#3: Protein | Mass: 10978.736 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
#4: Chemical | ChemComp-K / |
#5: Water | ChemComp-HOH / |
Has ligand of interest | Y |
Nonpolymer details | The potassium ion observed in the structure came from the buffer used during gel filtration ...The potassium ion observed in the structure came from the buffer used during gel filtration purification of KcsA. No potassium was present in the solution used to soak the crystal |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.91 Å3/Da / Density % sol: 68.56 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 5.5 Details: 20 % PEG 400, 50 mM Magnesium Acetate, 50 mM Sodium Acetate, pH 5.5 |
-Data collection
Diffraction | Mean temperature: 98 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Oct 28, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9791 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→67.84 Å / Num. obs: 35095 / % possible obs: 99.9 % / Redundancy: 6.9 % / CC1/2: 0.99 / Net I/σ(I): 7.6 |
Reflection shell | Resolution: 2.4→2.49 Å / Num. unique obs: 3680 / CC1/2: 0.11 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1k4c Resolution: 2.4→67.84 Å / Cor.coef. Fo:Fc: 0.927 / Cor.coef. Fo:Fc free: 0.923 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.288 / ESU R Free: 0.229 Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 120.51 Å2 / Biso mean: 34.769 Å2 / Biso min: 16.57 Å2
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Refinement step | Cycle: final / Resolution: 2.4→67.84 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.4→2.462 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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