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- PDB-3f8r: Crystal structure of Sulfolobus solfataricus Thioredoxin reductas... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3f8r | ||||||
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Title | Crystal structure of Sulfolobus solfataricus Thioredoxin reductase B3 in complex with two NADP molecules | ||||||
![]() | Thioredoxin reductase (TrxB-3) | ||||||
![]() | OXIDOREDUCTASE / Redox protein / nucleotide binding / FAD / Flavoprotein | ||||||
Function / homology | ![]() NADH dehydrogenase / thioredoxin-disulfide reductase / thioredoxin-disulfide reductase (NADPH) activity / removal of superoxide radicals / cell redox homeostasis / nucleotide binding / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Ruggiero, A. / Masullo, M. / Ruocco, M.R. / Arcari, P. / Zagari, A. / Vitagliano, L. | ||||||
![]() | ![]() Title: Structure and stability of a thioredoxin reductase from Sulfolobus solfataricus: a thermostable protein with two functions Authors: Ruggiero, A. / Masullo, M. / Ruocco, M.R. / Grimaldi, P. / Lanzotti, M.A. / Arcari, P. / Zagari, A. / Vitagliano, L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 254.9 KB | Display | ![]() |
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PDB format | ![]() | 207.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 2.7 MB | Display | ![]() |
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Full document | ![]() | 2.7 MB | Display | |
Data in XML | ![]() | 52.8 KB | Display | |
Data in CIF | ![]() | 71 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3f8dC ![]() 3f8pSC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 35266.676 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: Q97W27, UniProt: Q8X236*PLUS, EC: 1.6.4.5 #2: Chemical | ChemComp-NAP / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.14 Å3/Da / Density % sol: 42.6 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion / pH: 7.5 Details: 12-15% (w/v) poly-ethylene glycol 2000 monomethyl ether (PEG mmE 2000), 0.1M (NH4)2SO4, 50 mM sodium acetate (pH 4.6), Protein 4-9 mg/ml, protein/NADP=1/100, VAPOR DIFFUSION, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Dec 8, 2003 |
Radiation | Monochromator: Si 111 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9756 Å / Relative weight: 1 |
Reflection | Resolution: 1.95→20 Å / Num. all: 80365 / Num. obs: 80365 / % possible obs: 89.9 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Rmerge(I) obs: 0.053 |
Reflection shell | Resolution: 1.95→2.02 Å / Rmerge(I) obs: 0.339 / % possible all: 73.5 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 3F8P Resolution: 1.95→15 Å / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 1.95→15 Å
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