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- PDB-3f8d: Structure of Sulfolobus solfataricus Thioredoxin reductase Mutant... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3f8d | ||||||
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Title | Structure of Sulfolobus solfataricus Thioredoxin reductase Mutant C147A | ||||||
![]() | Thioredoxin reductase (TrxB-3) | ||||||
![]() | OXIDOREDUCTASE / Redox protein / nucleotide binding / FAD / Flavoprotein | ||||||
Function / homology | ![]() NADH dehydrogenase / thioredoxin-disulfide reductase / thioredoxin-disulfide reductase (NADPH) activity / removal of superoxide radicals / cell redox homeostasis / nucleotide binding / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Ruggiero, A. / Masullo, M. / Ruocco, M.R. / Arcari, P. / Zagari, A. / Vitagliano, L. | ||||||
![]() | ![]() Title: Structure and stability of a thioredoxin reductase from Sulfolobus solfataricus: a thermostable protein with two functions Authors: Ruggiero, A. / Masullo, M. / Ruocco, M.R. / Grimaldi, P. / Lanzotti, M.A. / Arcari, P. / Zagari, A. / Vitagliano, L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 253.6 KB | Display | ![]() |
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PDB format | ![]() | 203.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.4 MB | Display | ![]() |
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Full document | ![]() | 1.4 MB | Display | |
Data in XML | ![]() | 48.7 KB | Display | |
Data in CIF | ![]() | 67.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3f8pSC ![]() 3f8rC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 35234.613 Da / Num. of mol.: 4 / Mutation: C147A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: Q97W27, UniProt: Q8X236*PLUS, EC: 1.6.4.5 #2: Chemical | ChemComp-FAD / #3: Chemical | ChemComp-SO4 / #4: Chemical | ChemComp-ACY / | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.1 Å3/Da / Density % sol: 41.43 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion / pH: 7.5 Details: 12-15% (w/v) poly-ethylene glycol 2000 monomethyl ether, 0.1M (NH4)2SO4, 50mM sodium acetate (pH 4.6), protein 4-9 mg/ml, VAPOR DIFFUSION, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Dec 8, 2003 |
Radiation | Monochromator: Monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9756 Å / Relative weight: 1 |
Reflection | Resolution: 1.4→30 Å / Num. all: 231790 / Num. obs: 231790 / % possible obs: 98.9 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Rmerge(I) obs: 0.081 |
Reflection shell | Resolution: 1.4→1.45 Å / Rmerge(I) obs: 0.326 / % possible all: 91.7 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 3F8P Resolution: 1.4→15 Å / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 1.4→15 Å
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