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- PDB-3f3f: Crystal structure of the nucleoporin pair Nup85-Seh1, space group P21 -

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Basic information

Entry
Database: PDB / ID: 3f3f
TitleCrystal structure of the nucleoporin pair Nup85-Seh1, space group P21
Components
  • Nucleoporin NUP85
  • Nucleoporin SEH1
KeywordsSTRUCTURAL PROTEIN / Protein Complex / Nucleoporin / Nucleoporin Complex / Nuclear Pore Complex / Macromolecular Assembly / Membrane Coat / Nucleocytoplasmic Transport / beta-propeller / solenoid domain / mRNA transport / Nucleus / Protein transport / Translocation / WD repeat
Function / homology
Function and homology information


Seh1-associated complex / nuclear pore localization / regulation of TORC1 signaling / nuclear pore outer ring / structural constituent of nuclear pore / nucleocytoplasmic transport / vacuolar membrane / ribosomal large subunit export from nucleus / mRNA transport / mRNA export from nucleus ...Seh1-associated complex / nuclear pore localization / regulation of TORC1 signaling / nuclear pore outer ring / structural constituent of nuclear pore / nucleocytoplasmic transport / vacuolar membrane / ribosomal large subunit export from nucleus / mRNA transport / mRNA export from nucleus / nuclear pore / positive regulation of TORC1 signaling / cellular response to amino acid starvation / protein import into nucleus / protein transport / nuclear envelope / nuclear membrane / positive regulation of DNA-templated transcription
Similarity search - Function
N-terminal domain of TfIIb - #500 / Nucleoporin Nup85-like / Nup85 Nucleoporin / Sec13/Seh1 family / N-terminal domain of TfIIb / YVTN repeat-like/Quinoprotein amine dehydrogenase / 7 Propeller / Methylamine Dehydrogenase; Chain H / Single Sheet / WD domain, G-beta repeat ...N-terminal domain of TfIIb - #500 / Nucleoporin Nup85-like / Nup85 Nucleoporin / Sec13/Seh1 family / N-terminal domain of TfIIb / YVTN repeat-like/Quinoprotein amine dehydrogenase / 7 Propeller / Methylamine Dehydrogenase; Chain H / Single Sheet / WD domain, G-beta repeat / WD40 repeats / WD40 repeat / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily / Mainly Beta
Similarity search - Domain/homology
Nucleoporin NUP85 / Nucleoporin SEH1
Similarity search - Component
Biological speciesSaccharomyces cerevisiae (brewer's yeast)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MIRAS / Resolution: 2.9 Å
AuthorsDebler, E.W. / Hseo, H. / Ma, Y. / Blobel, G. / Hoelz, A.
CitationJournal: Mol.Cell / Year: 2008
Title: A fence-like coat for the nuclear pore membrane.
Authors: Debler, E.W. / Ma, Y. / Seo, H.S. / Hsia, K.C. / Noriega, T.R. / Blobel, G. / Hoelz, A.
History
DepositionOct 30, 2008Deposition site: RCSB / Processing site: RCSB
Revision 1.0Apr 7, 2009Provider: repository / Type: Initial release
Revision 1.1Jul 13, 2011Group: Version format compliance
Revision 1.2Dec 27, 2023Group: Data collection / Database references
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / struct_ref_seq_dif
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ref_seq_dif.details

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Nucleoporin SEH1
B: Nucleoporin SEH1
C: Nucleoporin NUP85
D: Nucleoporin NUP85
E: Nucleoporin SEH1
F: Nucleoporin SEH1
G: Nucleoporin NUP85
H: Nucleoporin NUP85


Theoretical massNumber of molelcules
Total (without water)417,3418
Polymers417,3418
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area36810 Å2
ΔGint-186.9 kcal/mol
Surface area128910 Å2
MethodPISA
Unit cell
Length a, b, c (Å)79.840, 166.187, 188.904
Angle α, β, γ (deg.)90.00, 93.02, 90.00
Int Tables number4
Space group name H-MP1211
DetailsThe biological unit is the complete heterooctamer that is present in the asymmetric unit

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Components

#1: Protein
Nucleoporin SEH1 / Nuclear pore protein SEH1 / SEC13 homolog 1


Mass: 39406.016 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast)
Gene: SEH1 / Plasmid: pETDuet-1 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21-CodonPlus(DE3)-RIL / References: UniProt: P53011
#2: Protein
Nucleoporin NUP85 / Nuclear pore protein NUP85


Mass: 64929.242 Da / Num. of mol.: 4 / Fragment: UNP residues 1-570
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast)
Gene: NUP85, RAT9 / Plasmid: pETDuet-1 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21-CodonPlus(DE3)-RIL / References: UniProt: P46673

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3 Å3/Da / Density % sol: 58.98 %
Crystal growTemperature: 298 K / Method: vapor diffusion, hanging drop / pH: 6.1
Details: PEG 10000, MES buffer, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: ALS / Beamline: 8.2.2 / Wavelength: 1 Å
DetectorType: ADSC QUANTUM 315 / Detector: CCD / Date: May 8, 2008
RadiationMonochromator: Si(111) double crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.9→50 Å / Num. all: 111202 / Num. obs: 108644 / % possible obs: 97.7 % / Observed criterion σ(I): -3 / Redundancy: 6.7 % / Rsym value: 0.079 / Net I/σ(I): 19.9
Reflection shellResolution: 2.9→2.97 Å / Redundancy: 5 % / Mean I/σ(I) obs: 1.8 / Num. unique all: 7406 / Rsym value: 0.079 / % possible all: 78

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Processing

Software
NameVersionClassification
ADSCQuantumdata collection
SHARPphasing
CNS1.2refinement
HKL-2000data reduction
SCALEPACKdata scaling
RefinementMethod to determine structure: MIRAS / Resolution: 2.9→50 Å / Stereochemistry target values: Engh & Huber
RfactorNum. reflection
Rfree0.265 9722
Rwork0.246 -
obs0.246 97295
all-108554
Refinement stepCycle: LAST / Resolution: 2.9→50 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms25239 0 0 0 25239
Refine LS restraints
Refine-IDTypeDev ideal
X-RAY DIFFRACTIONc_bond_d0.009
X-RAY DIFFRACTIONc_angle_deg1.6

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