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- PDB-3ejl: Replacement of Val3 in Human Thymidylate Synthase Affects Its Kin... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3ejl | ||||||
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Title | Replacement of Val3 in Human Thymidylate Synthase Affects Its Kinetic Properties and Intracellular Stability | ||||||
![]() | Thymidylate synthase | ||||||
![]() | TRANSFERASE / Methyltransferase / Nucleotide biosynthesis | ||||||
Function / homology | ![]() uracil metabolic process / response to organophosphorus / intestinal epithelial cell maturation / response to folic acid / Interconversion of nucleotide di- and triphosphates / thymidylate synthase / response to vitamin A / sequence-specific mRNA binding / cartilage development / tetrahydrofolate interconversion ...uracil metabolic process / response to organophosphorus / intestinal epithelial cell maturation / response to folic acid / Interconversion of nucleotide di- and triphosphates / thymidylate synthase / response to vitamin A / sequence-specific mRNA binding / cartilage development / tetrahydrofolate interconversion / thymidylate synthase activity / folic acid binding / dTMP biosynthetic process / dTTP biosynthetic process / DNA biosynthetic process / G1/S-Specific Transcription / developmental growth / dihydrofolate reductase activity / response to glucocorticoid / mRNA regulatory element binding translation repressor activity / response to cytokine / response to progesterone / liver regeneration / response to toxic substance / circadian rhythm / methylation / response to ethanol / mitochondrial inner membrane / negative regulation of translation / mitochondrial matrix / response to xenobiotic stimulus / protein homodimerization activity / mitochondrion / nucleus / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Huang, X. / Gibson, L.M. / Bell, B.J. / Lovelace, L.L. / Pena, M.M. / Berger, F.G. / Berger, S.H. | ||||||
![]() | ![]() Title: Replacement of Val3 in human thymidylate synthase affects its kinetic properties and intracellular stability . Authors: Huang, X. / Gibson, L.M. / Bell, B.J. / Lovelace, L.L. / Pena, M.M. / Berger, F.G. / Berger, S.H. / Lebioda, L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 231.7 KB | Display | ![]() |
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PDB format | ![]() | 194.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 720.8 KB | Display | ![]() |
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Full document | ![]() | 810.9 KB | Display | |
Data in XML | ![]() | 53 KB | Display | |
Data in CIF | ![]() | 70.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3eawC ![]() 3ebuC ![]() 3ed7C ![]() 3edwC ![]() 3ef9C ![]() 3gg5C ![]() 3gh0C ![]() 3gh2C C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 35810.980 Da / Num. of mol.: 4 / Mutation: V3F Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | #3: Chemical | ChemComp-UFP / | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.9 Å3/Da / Density % sol: 57.58 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 8 Details: 0.1M Tris,PH8.0, 30mM ammonium sulfate, 24%PEG4000, 20mM BME, pH 8.0 , VAPOR DIFFUSION, HANGING DROP, temperature 277K |
-Data collection
Diffraction source | Source: ![]() ![]() ![]() |
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Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Jun 28, 2008 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 2.75→50 Å / Num. obs: 27289 / % possible obs: 78.5 % / Redundancy: 2.9 % / Rmerge(I) obs: 0.085 / Χ2: 2.738 / Net I/σ(I): 22.408 |
Reflection shell | Resolution: 2.75→2.85 Å / Redundancy: 1.2 % / Rmerge(I) obs: 0.457 / Num. unique all: 1139 / Χ2: 4.234 / % possible all: 26.4 |
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Processing
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Refinement | Resolution: 3.2→50 Å / Occupancy max: 1 / Occupancy min: 1 / σ(F): 0
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Solvent computation | Bsol: 82.877 Å2 | ||||||||||||||||||||||||||||
Displacement parameters | Biso max: 159.87 Å2 / Biso mean: 91.477 Å2 / Biso min: 15.37 Å2
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Refinement step | Cycle: LAST / Resolution: 3.2→50 Å
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Refine LS restraints |
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Xplor file |
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