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- PDB-3cpp: CRYSTAL STRUCTURE OF THE CARBON MONOXY-SUBSTRATE-CYTOCHROME P450-... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3cpp | ||||||
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Title | CRYSTAL STRUCTURE OF THE CARBON MONOXY-SUBSTRATE-CYTOCHROME P450-CAM TERNARY COMPLEX | ||||||
![]() | CYTOCHROME P450-CAM | ||||||
![]() | OXIDOREDUCTASE(OXYGENASE) | ||||||
Function / homology | ![]() camphor 5-monooxygenase / camphor 5-monooxygenase activity / (+)-camphor catabolic process / cholest-4-en-3-one 26-monooxygenase activity / steroid hydroxylase activity / cholesterol catabolic process / iron ion binding / heme binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
![]() | Raag, R. / Poulos, T.L. | ||||||
![]() | ![]() Title: Crystal structure of the carbon monoxide-substrate-cytochrome P-450CAM ternary complex. Authors: Raag, R. / Poulos, T.L. #1: ![]() Title: The Structural Basis for Substrate-Induced Changes in Redox Potential and Spin Equilibrium in Cytochrome P450-Cam Authors: Raag, R. / Poulos, T.L. #2: ![]() Title: High-Resolution Crystal Structure of Cytochrome P450-Cam Authors: Poulos, T.L. / Finzel, B.C. / Howard, A.J. #3: ![]() Title: The 2.6-Angstroms Crystal Structure of Pseudomonas Putida Cytochrome P450 Authors: Poulos, T.L. / Finzel, B.C. / Gunsalus, I.C. / Wagner, G.C. / Kraut, J. #4: ![]() Title: Crystal Structure of Substrate-Free Pseudomonas Putida Cytochrome P450 Authors: Poulos, T.L. / Finzel, B.C. / Howard, A.J. #5: ![]() Title: Heme Enzyme Structure and Function Authors: Poulos, T.L. / Finzel, B.C. #6: ![]() Title: Preliminary Crystallographic Data on Cytochrome P450-Cam Authors: Poulos, T.L. / Perez, M. / Wagner, G.C. #7: ![]() Title: The Primary Structure of the Monoxygenase Cytochrome P450-Cam Authors: Haniu, M. / Armes, L.G. / Tanaka, M. / Yasunobu, K.T. / Shastry, B.S. / Wagner, G.C. / Gunsalus, I.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 100 KB | Display | ![]() |
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PDB format | ![]() | 76.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 501.5 KB | Display | ![]() |
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Full document | ![]() | 513.9 KB | Display | |
Data in XML | ![]() | 11.7 KB | Display | |
Data in CIF | ![]() | 18.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Atom site foot note | 1: RESIDUES PRO 89, PRO 100, PRO 106 ARE CIS PROLINES. |
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Components
#1: Protein | Mass: 46588.879 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() |
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#2: Chemical | ChemComp-HEM / |
#3: Chemical | ChemComp-CMO / |
#4: Chemical | ChemComp-CAM / |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.2 Å3/Da / Density % sol: 44.16 % | |||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 7 / Method: other | |||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Reflection | *PLUS Highest resolution: 1.9 Å / Lowest resolution: 2.2 Å / Num. measured all: 73981 / Rmerge(I) obs: 0.072 |
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Processing
Software | Name: PROFFT / Classification: refinement | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Rfactor obs: 0.19 / Highest resolution: 1.9 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 1.9 Å
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Refine LS restraints |
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Refinement | *PLUS Lowest resolution: 10 Å / Num. reflection all: 24228 / σ(I): 2 / Highest resolution: 1.9 Å / Num. reflection obs: 18785 / Rfactor obs: 0.19 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: p_dihedral_angle_d / Dev ideal: 0.033 |