+Open data
-Basic information
Entry | Database: PDB / ID: 3com | ||||||
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Title | Crystal structure of Mst1 kinase | ||||||
Components | Serine/threonine-protein kinase 4Serine/threonine-specific protein kinase | ||||||
Keywords | TRANSFERASE / kinase / Mst1 / Serine/threonine-protein kinase 4 / STE20-like kinase / PSI / structural genomics / Protein Structure Initiative / New York SGX Research Center for Structural Genomics / NYSGXRC / Alternative splicing / Apoptosis / ATP-binding / Coiled coil / Cytoplasm / Magnesium / Metal-binding / Nucleotide-binding / Nucleus / Phosphoprotein / Polymorphism | ||||||
Function / homology | Function and homology information positive regulation of hepatocyte apoptotic process / cell differentiation involved in embryonic placenta development / regulation of cell differentiation involved in embryonic placenta development / primitive hemopoiesis / neural tube formation / positive regulation of extrinsic apoptotic signaling pathway via death domain receptors / positive regulation of substrate-dependent cell migration, cell attachment to substrate / endocardium development / negative regulation of organ growth / hippo signaling ...positive regulation of hepatocyte apoptotic process / cell differentiation involved in embryonic placenta development / regulation of cell differentiation involved in embryonic placenta development / primitive hemopoiesis / neural tube formation / positive regulation of extrinsic apoptotic signaling pathway via death domain receptors / positive regulation of substrate-dependent cell migration, cell attachment to substrate / endocardium development / negative regulation of organ growth / hippo signaling / Signaling by Hippo / organ growth / branching involved in blood vessel morphogenesis / hepatocyte apoptotic process / regulation of MAPK cascade / extrinsic apoptotic signaling pathway via death domain receptors / canonical Wnt signaling pathway / positive regulation of fat cell differentiation / keratinocyte differentiation / protein serine/threonine kinase activator activity / epithelial cell proliferation / central nervous system development / protein tetramerization / negative regulation of canonical Wnt signaling pathway / cell morphogenesis / protein import into nucleus / negative regulation of epithelial cell proliferation / positive regulation of peptidyl-serine phosphorylation / positive regulation of protein binding / peptidyl-serine phosphorylation / RNA polymerase II-specific DNA-binding transcription factor binding / protein autophosphorylation / protein stabilization / nuclear body / non-specific serine/threonine protein kinase / protein kinase activity / intracellular signal transduction / positive regulation of protein phosphorylation / positive regulation of apoptotic process / protein phosphorylation / protein serine kinase activity / protein serine/threonine kinase activity / apoptotic process / magnesium ion binding / signal transduction / protein homodimerization activity / protein-containing complex / nucleoplasm / ATP binding / identical protein binding / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.2 Å | ||||||
Authors | Atwell, S. / Burley, S.K. / Dickey, M. / Leon, B. / Sauder, J.M. / New York SGX Research Center for Structural Genomics (NYSGXRC) | ||||||
Citation | Journal: To be Published Title: Crystal structure of Mst1 kinase. Authors: Atwell, S. / Burley, S.K. / Dickey, M. / Leon, B. / Sauder, J.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3com.cif.gz | 122.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3com.ent.gz | 95.4 KB | Display | PDB format |
PDBx/mmJSON format | 3com.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/co/3com ftp://data.pdbj.org/pub/pdb/validation_reports/co/3com | HTTPS FTP |
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-Related structure data
Related structure data | 3ckwS S: Starting model for refinement |
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Similar structure data | |
Other databases |
-Links
-Assembly
Deposited unit |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 35793.863 Da / Num. of mol.: 2 / Fragment: Protein kinase domain: Residues 2-311 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: STK4, MST1 / Plasmid: pSGX4 / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) References: UniProt: Q13043, non-specific serine/threonine protein kinase #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.05 Å3/Da / Density % sol: 59.74 % |
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Crystal grow | Temperature: 294 K / Method: vapor diffusion, sitting drop / pH: 10.5 Details: 1700mM Ammonium sulfate, 300mM Lithium sulfate, 100mM CAPS pH 10.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 31-ID / Wavelength: 0.9794 Å |
Detector | Type: MAR CCD 165 mm / Detector: CCD / Date: Jun 1, 2004 / Details: KV mirrors |
Radiation | Monochromator: Kohzu 111 diamond / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9794 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→30 Å / Num. obs: 40513 / % possible obs: 90.2 % / Redundancy: 3.2 % / Biso Wilson estimate: 27.2 Å2 / Rmerge(I) obs: 0.118 / Net I/σ(I): 6.5 |
Reflection shell | Resolution: 2.2→2.32 Å / Redundancy: 3.1 % / Rmerge(I) obs: 0.439 / Mean I/σ(I) obs: 2.7 / Num. unique all: 6082 / % possible all: 93.9 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB entry 3CKW Resolution: 2.2→30 Å / Isotropic thermal model: Isotropic / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.2→30 Å
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Refine LS restraints |
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