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Open data
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Basic information
Entry | Database: PDB / ID: 3cn6 | ||||||
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Title | Crystal structure of the Spinach Aquaporin SoPIP2;1 S274E mutant | ||||||
![]() | Aquaporin | ||||||
![]() | TRANSPORT PROTEIN / membrane protein / aquaporin / Transmembrane / Transport | ||||||
Function / homology | ![]() water channel activity / plasmodesma / vacuole / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Nyblom, M. / Alfredsson, A. / Hallgren, K. / Hedfalk, K. / Neutze, R. / Tornroth-Horsefield, S. | ||||||
![]() | ![]() Title: Structural and functional analysis of SoPIP2;1 mutants adds insight into plant aquaporin gating. Authors: Nyblom, M. / Frick, A. / Wang, Y. / Ekvall, M. / Hallgren, K. / Hedfalk, K. / Neutze, R. / Tajkhorshid, E. / Tornroth-Horsefield, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 104.8 KB | Display | ![]() |
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PDB format | ![]() | 80.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 433.1 KB | Display | ![]() |
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Full document | ![]() | 443.4 KB | Display | |
Data in XML | ![]() | 19.8 KB | Display | |
Data in CIF | ![]() | 26.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 32600.793 Da / Num. of mol.: 2 / Mutation: S274E Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.91 Å3/Da / Density % sol: 57.74 % |
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Crystal grow | Temperature: 280 K / Method: vapor diffusion, hanging drop / pH: 7 Details: Reservoir: 0.1M Tris, 0.2M NaCl, 24% PEG400. Reservoir mixed 1:4 with 0.1M CdCl2, and then mixed 1:1 with protein, centrifuged briefly and supernatant used for crystallization, pH 7, VAPOR ...Details: Reservoir: 0.1M Tris, 0.2M NaCl, 24% PEG400. Reservoir mixed 1:4 with 0.1M CdCl2, and then mixed 1:1 with protein, centrifuged briefly and supernatant used for crystallization, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 280K |
-Data collection
Diffraction source | Source: ![]() ![]() ![]() |
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Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Sep 8, 2007 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.931 Å / Relative weight: 1 |
Reflection | Resolution: 2.95→34.86 Å / Num. all: 15560 / Num. obs: 15560 / % possible obs: 100 % / Redundancy: 4 % / Rsym value: 0.155 |
Reflection shell | Resolution: 2.95→3.11 Å / Redundancy: 4.2 % / Num. unique all: 2274 / Rsym value: 0.33 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 32.609 Å2
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Refinement step | Cycle: LAST / Resolution: 2.95→34.86 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.95→3.026 Å / Total num. of bins used: 20
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