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Open data
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Basic information
Entry | Database: PDB / ID: 3cbm | ||||||
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Title | SET7/9-ER-AdoMet complex | ||||||
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![]() | TRANSFERASE/TRANSFERASE RECEPTOR / Estrogen receptor / protein lysine methylation / Activator / Chromatin regulator / Chromosomal protein / Methyltransferase / Nucleus / S-adenosyl-L-methionine / Transcription / Transcription regulation / Transferase / Alternative splicing / DNA-binding / Lipid-binding / Metal-binding / Phosphoprotein / Polymorphism / Steroid-binding / Zinc / Zinc-finger / TRANSFERASE-TRANSFERASE RECEPTOR COMPLEX | ||||||
Function / homology | ![]() peptidyl-lysine monomethylation / peptidyl-lysine dimethylation / [histone H3]-lysine4 N-methyltransferase / histone H3K4 monomethyltransferase activity / protein-lysine N-methyltransferase activity / histone H3 methyltransferase activity / histone methyltransferase activity / heterochromatin organization / PKMTs methylate histone lysines / p53 binding ...peptidyl-lysine monomethylation / peptidyl-lysine dimethylation / [histone H3]-lysine4 N-methyltransferase / histone H3K4 monomethyltransferase activity / protein-lysine N-methyltransferase activity / histone H3 methyltransferase activity / histone methyltransferase activity / heterochromatin organization / PKMTs methylate histone lysines / p53 binding / chromatin organization / chromosome / response to ethanol / DNA damage response / chromatin binding / nucleolus / positive regulation of DNA-templated transcription / nucleoplasm / nucleus Similarity search - Function | ||||||
Biological species | ![]() synthetic construct (others) | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Cheng, X. / Jia, D. | ||||||
![]() | ![]() Title: Regulation of estrogen receptor alpha by the SET7 lysine methyltransferase. Authors: Subramanian, K. / Jia, D. / Kapoor-Vazirani, P. / Powell, D.R. / Collins, R.E. / Sharma, D. / Peng, J. / Cheng, X. / Vertino, P.M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 71 KB | Display | ![]() |
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PDB format | ![]() | 50 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 836.3 KB | Display | ![]() |
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Full document | ![]() | 839.8 KB | Display | |
Data in XML | ![]() | 14 KB | Display | |
Data in CIF | ![]() | 19.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3cboC ![]() 3cbpC ![]() 1o9sS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 28563.785 Da / Num. of mol.: 1 / Fragment: UNP residues 111-366 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: Q8WTS6, histone-lysine N-methyltransferase | ||
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#2: Protein/peptide | Mass: 1161.419 Da / Num. of mol.: 1 / Fragment: UNP residues 298-307 / Source method: obtained synthetically / Details: ER peptide / Source: (synth.) synthetic construct (others) / ![]() | ||
#3: Chemical | ChemComp-SAH / | ||
#4: Chemical | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.23 Å3/Da / Density % sol: 44.88 % |
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Crystal grow | Method: vapor diffusion / pH: 8 Details: 40-42.5% PEG3350, 100 mM Tris, pH 8.0, VAPOR DIFFUSION |
-Data collection
Diffraction source | Source: ![]() ![]() ![]() |
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Detector | Type: MAR CCD 165 mm / Detector: CCD / Date: Apr 7, 2006 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.69→25.59 Å / Num. obs: 27719 / % possible obs: 90.4 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Biso Wilson estimate: 20.2 Å2 / Rmerge(I) obs: 0.068 / Net I/σ(I): 16.8 |
Reflection shell | Resolution: 1.69→1.75 Å / Rmerge(I) obs: 0.571 / Mean I/σ(I) obs: 3.4 / Num. unique all: 2379 / % possible all: 83.7 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1O9S Resolution: 1.69→25.59 Å / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber Details: BULK SOLVENT MODELING. METHOD USED : FLAT MODEL KSOL : 0.366634 BSOL : 44.8929 (A**2)
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Displacement parameters | Biso mean: 27.4 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.69→25.59 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.69→1.75 Å / Rfactor Rfree error: 0.024
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