+Open data
-Basic information
Entry | Database: PDB / ID: 3alp | ||||||
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Title | Cell adhesion protein | ||||||
Components | Poliovirus receptor-related protein 1 | ||||||
Keywords | CELL ADHESION / immunoglobulin-like domain / V-set / C2-set | ||||||
Function / homology | Function and homology information desmosome organization / Nectin/Necl trans heterodimerization / protein localization to cell junction / lens morphogenesis in camera-type eye / growth cone membrane / enamel mineralization / cell-cell contact zone / Adherens junctions interactions / virion binding / heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules ...desmosome organization / Nectin/Necl trans heterodimerization / protein localization to cell junction / lens morphogenesis in camera-type eye / growth cone membrane / enamel mineralization / cell-cell contact zone / Adherens junctions interactions / virion binding / heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules / regulation of synapse assembly / apical junction complex / homophilic cell adhesion via plasma membrane adhesion molecules / coreceptor activity / presynaptic active zone membrane / hippocampal mossy fiber to CA3 synapse / cell adhesion molecule binding / axon guidance / adherens junction / cell-cell adhesion / virus receptor activity / retina development in camera-type eye / iron ion transport / carbohydrate binding / cell adhesion / immune response / symbiont entry into host cell / dendrite / protein-containing complex binding / protein homodimerization activity / extracellular region / membrane / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 2.804 Å | ||||||
Authors | Narita, H. / Nakagawa, A. / Suzuki, M. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2011 Title: Crystal Structure of the cis-Dimer of Nectin-1: implications for the architecture of cell-cell junctions Authors: Narita, H. / Yamamoto, Y. / Suzuki, M. / Miyazaki, N. / Yoshida, A. / Kawai, K. / Iwasaki, K. / Nakagawa, A. / Takai, Y. / Sakisaka, T. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3alp.cif.gz | 247.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3alp.ent.gz | 209.4 KB | Display | PDB format |
PDBx/mmJSON format | 3alp.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/al/3alp ftp://data.pdbj.org/pub/pdb/validation_reports/al/3alp | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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Unit cell |
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-Components
#1: Protein | Mass: 36938.492 Da / Num. of mol.: 2 / Fragment: extracellular region (UNP RESIDUES 30-335) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: HVEC, PRR1, PVRL1 / Plasmid: pET21b(+) / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q15223 #2: Chemical | #3: Chemical | ChemComp-HEZ / | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 5.06 Å3/Da / Density % sol: 75.69 % |
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Crystal grow | Temperature: 296 K / Method: vapor diffusion, hanging drop / pH: 5 Details: 1% PEG 3350, 50mM Bis-Tris propane, 50mM citric acid, 6% hexane-1,6-diol , VAPOR DIFFUSION, HANGING DROP, temperature 296K |
-Data collection
Diffraction |
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Diffraction source |
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Detector |
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Radiation |
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Radiation wavelength |
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Reflection | Resolution: 2.8→50 Å / Num. obs: 36109 / % possible obs: 97.9 % / Redundancy: 3.8 % / Rmerge(I) obs: 0.068 / Net I/σ(I): 19.4 | ||||||||||||||||||
Reflection shell | Resolution: 2.8→2.9 Å / Redundancy: 3.9 % / Rmerge(I) obs: 0.358 / Mean I/σ(I) obs: 4.3 / Num. unique all: 13666 / % possible all: 96.9 |
-Processing
Software |
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Refinement | Method to determine structure: SAD / Resolution: 2.804→35.989 Å / SU ML: 0.32 / Cross valid method: THROUGHOUT / σ(F): 0.02 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 46.604 Å2 / ksol: 0.309 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 80.04 Å2
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Refinement step | Cycle: LAST / Resolution: 2.804→35.989 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 12
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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