+Open data
-Basic information
Entry | Database: PDB / ID: 3al7 | ||||||
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Title | Recombinant thaumatin I at 1.1 A | ||||||
Components | Thaumatin I | ||||||
Keywords | PLANT PROTEIN / THAUMATIN / SWEET-TASTING PROTEIN | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Thaumatococcus daniellii (katemfe) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.1 Å | ||||||
Authors | Masuda, T. / Mikami, B. / Kitabatake, N. | ||||||
Citation | Journal: Acta Crystallogr.,Sect.F / Year: 2011 Title: High-resolution structure of the recombinant sweet-tasting protein thaumatin I Authors: Masuda, T. / Ohta, K. / Mikami, B. / Kitabatake, N. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3al7.cif.gz | 157.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3al7.ent.gz | 127.6 KB | Display | PDB format |
PDBx/mmJSON format | 3al7.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/al/3al7 ftp://data.pdbj.org/pub/pdb/validation_reports/al/3al7 | HTTPS FTP |
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-Related structure data
Related structure data | 3aldC 1rqwS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 22228.043 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thaumatococcus daniellii (katemfe) / Plasmid: pPIC6-preTH / Production host: Pichia pastoris (fungus) / Strain (production host): X-33, SMD1168H / References: UniProt: Q8RVT0, UniProt: P02883*PLUS | ||||
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#2: Chemical | #3: Chemical | ChemComp-GOL / #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.8 Å3/Da / Density % sol: 56.13 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 0.02M ADA, 0.75M TARTRATE. 20%GLYCEROL, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SPring-8 / Beamline: BL38B1 / Wavelength: 0.7 |
Detector | Type: RIGAKU RAXIS V / Detector: IMAGE PLATE / Date: Apr 18, 2009 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.7 Å / Relative weight: 1 |
Reflection | Resolution: 1.1→20 Å / Num. obs: 102055 / % possible obs: 98.9 % / Redundancy: 6.5 % / Rmerge(I) obs: 0.084 / Net I/σ(I): 27.69 |
Reflection shell | Resolution: 1.1→1.14 Å / Redundancy: 4.2 % / Rmerge(I) obs: 0.368 / Mean I/σ(I) obs: 2.2 / % possible all: 90.8 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1RQW Resolution: 1.1→10 Å / Num. parameters: 20658 / Num. restraintsaints: 27036 / Cross valid method: FREE R / σ(F): 0 / Stereochemistry target values: Engh & Huber / Details: ANISOTROPIC REFINEMENT REDUCED FREE R (NO CUTOFF)
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Refine analyze | Num. disordered residues: 62 / Occupancy sum hydrogen: 1426.22 / Occupancy sum non hydrogen: 2056.34 | |||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.1→10 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.1→1.15 Å
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