HOWEVER, SINCE MANY CONFLICTS ROSE UP DURING REFINEMENT, THE DEPOSITORS BUILT THIS STRUCTURE BASED ...HOWEVER, SINCE MANY CONFLICTS ROSE UP DURING REFINEMENT, THE DEPOSITORS BUILT THIS STRUCTURE BASED ON THE ELECTRON DENSITY INSTEAD. THEY THINK THE DNA SEQUENCE IN Q02402_9FUNG COMES FROM ONE OF THE ISOZYME OF THE ANALYZED PROTEIN.
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 1
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試料調製
結晶
マシュー密度: 3.77 Å3/Da / 溶媒含有率: 67.37 %
結晶化
温度: 293 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 8.5 詳細: 0.2M trisodium citrate dihydrate, 0.1M Tris buffer pH 8.5, 30% polyethylene glycol 400, VAPOR DIFFUSION, HANGING DROP, temperature 293K
解像度: 2→24.16 Å / Cor.coef. Fo:Fc: 0.965 / Cor.coef. Fo:Fc free: 0.953 / SU B: 2.067 / SU ML: 0.06 / 交差検証法: THROUGHOUT / ESU R: 0.109 / ESU R Free: 0.104 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
反射数
%反射
Selection details
Rfree
0.17008
2291
5 %
RANDOM
Rwork
0.14186
43101
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obs
0.1433
45392
99.96 %
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK