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Open data
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Basic information
Entry | Database: PDB / ID: 3a28 | ||||||
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Title | Crystal structure of L-2,3-butanediol dehydrogenase | ||||||
![]() | L-2.3-butanediol dehydrogenase | ||||||
![]() | OXIDOREDUCTASE / chiral substrate recognition | ||||||
Function / homology | ![]() (S,S)-butanediol dehydrogenase / acetoin metabolic process / (S,S)-butanediol dehydrogenase activity / diacetyl reductase [(S)-acetoin forming] / diacetyl reductase ((S)-acetoin forming) (NAD+) activity / butanediol metabolic process / acetoin catabolic process / NADH binding / NAD+ binding / protein homotetramerization Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Otagiri, M. / Kurisu, G. / Ui, S. / Kusunoki, M. | ||||||
![]() | ![]() Title: Structural basis for chiral substrate recognition by two 2,3-butanediol dehydrogenases Authors: Otagiri, M. / Ui, S. / Takusagawa, Y. / Ohtsuki, T. / Kurisu, G. / Kusunoki, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 420.1 KB | Display | ![]() |
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PDB format | ![]() | 343 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 2.8 MB | Display | ![]() |
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Full document | ![]() | 2.9 MB | Display | |
Data in XML | ![]() | 99.4 KB | Display | |
Data in CIF | ![]() | 134 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1gegS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 27128.635 Da / Num. of mol.: 8 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: C-1012 / Production host: ![]() ![]() #2: Chemical | ChemComp-NAD / #3: Chemical | ChemComp-BME / #4: Chemical | ChemComp-MG / #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.25 Å3/Da / Density % sol: 45.41 % |
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Crystal grow | Method: vapor diffusion, hanging drop / Details: VAPOR DIFFUSION, HANGING DROP |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Dec 18, 2000 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2→40 Å / Num. obs: 121123 / % possible obs: 94.4 % / Rmerge(I) obs: 0.062 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1geg Resolution: 2→30 Å / Stereochemistry target values: Engh & Huber /
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Refinement step | Cycle: LAST / Resolution: 2→30 Å
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