[English] 日本語
![](img/lk-miru.gif)
- PDB-2zqq: Crystal structure of human AUH (3-methylglutaconyl-coa hydratase)... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 2zqq | ||||||
---|---|---|---|---|---|---|---|
Title | Crystal structure of human AUH (3-methylglutaconyl-coa hydratase) mixed with (AUUU)24A RNA | ||||||
![]() | Methylglutaconyl-CoA hydratase | ||||||
![]() | LYASE / BETA SPIRAL / Branched-chain amino acid catabolism / Disease mutation / Mitochondrion / RNA-binding / Transit peptide / Structural Genomics / NPPSFA / National Project on Protein Structural and Functional Analyses / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | ![]() methylglutaconyl-CoA hydratase / itaconyl-CoA hydratase / methylglutaconyl-CoA hydratase activity / itaconyl-CoA hydratase activity / L-leucine catabolic process / Branched-chain amino acid catabolism / enoyl-CoA hydratase activity / fatty acid beta-oxidation / mRNA 3'-UTR binding / mitochondrial matrix / mitochondrion Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kurimoto, K. / Kuwasako, K. / Muto, Y. / Nureki, O. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
![]() | ![]() Title: AU-rich RNA-binding induces changes in the quaternary structure of AUH Authors: Kurimoto, K. / Kuwasako, K. / Sandercock, A.M. / Unzai, S. / Robinson, C.V. / Muto, Y. / Yokoyama, S. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 304.8 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 249.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 467 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 497.1 KB | Display | |
Data in XML | ![]() | 61.5 KB | Display | |
Data in CIF | ![]() | 84.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2zqrC ![]() 1hzdS ![]() 1v4m ![]() 2e8k S: Starting model for refinement C: citing same article ( |
---|---|
Similar structure data | |
Other databases |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 29238.154 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.72 Å3/Da / Density % sol: 54.85 % |
---|---|
Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: DROP: 50MM HEPES, 1.0M AMMONIUM SULFATE, 1.0% PEG400, RESERVOIR: 100MM HEPES, 2.0M AMMONIUM SULFATE, 2.0% PEG400, pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Detector: CCD / Date: May 23, 2002 |
Radiation | Monochromator: SI111 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→50 Å / Num. obs: 88656 / % possible obs: 92.8 % / Observed criterion σ(I): 2 / Redundancy: 2.29 % / Rmerge(I) obs: 0.071 / Rsym value: 0.071 / Net I/σ(I): 23.6 |
Reflection shell | Resolution: 2.2→2.24 Å / Redundancy: 1.83 % / Rmerge(I) obs: 0.164 / Mean I/σ(I) obs: 4.725 / Rsym value: 0.156 / % possible all: 85.5 |
-
Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: 1HZD Resolution: 2.2→50 Å / σ(F): 0 / Stereochemistry target values: ENGH & HUBER
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.2→50 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 2.2→2.28 Å /
|