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- PDB-2zov: Structure of the periplasmic domain of MotB from Salmonella (crys... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2zov | ||||||
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Title | Structure of the periplasmic domain of MotB from Salmonella (crystal form I) | ||||||
![]() | Chemotaxis protein motB | ||||||
![]() | MEMBRANE PROTEIN / 2-layer sandwich / Bacterial flagellum / Cell projection / Chemotaxis / Flagellar rotation / Inner membrane / Membrane / Transmembrane | ||||||
Function / homology | ![]() archaeal or bacterial-type flagellum-dependent cell motility / chemotaxis / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Imada, K. / Kojima, S. / Namba, K. / Homma, M. | ||||||
![]() | ![]() Title: Stator assembly and activation mechanism of the flagellar motor by the periplasmic region of MotB Authors: Kojima, S. / Imada, K. / Sakuma, M. / Sudo, Y. / Kojima, C. / Minamino, T. / Homma, M. / Namba, K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 47.4 KB | Display | ![]() |
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PDB format | ![]() | 36.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 424.2 KB | Display | ![]() |
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Full document | ![]() | 429.6 KB | Display | |
Data in XML | ![]() | 11.2 KB | Display | |
Data in CIF | ![]() | 15.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 23982.191 Da / Num. of mol.: 1 / Fragment: C-terminal fragment 1, UNP Residues 88-291 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.63 Å3/Da / Density % sol: 53.27 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 4 Details: 6% PEG 4000, 0.1M Acetate, 0.22M Zn(OAc)2, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K | |||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | |||||||||||||||
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Apr 23, 2008 | |||||||||||||||
Radiation | Monochromator: double-crystal monochromator / Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||
Radiation wavelength |
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Reflection | Resolution: 2→33 Å / Num. all: 17248 / Num. obs: 17162 / % possible obs: 99.5 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 9.7 % / Biso Wilson estimate: 20.2 Å2 / Rsym value: 0.065 / Net I/σ(I): 22.1 | |||||||||||||||
Reflection shell | Resolution: 2→2.11 Å / Redundancy: 9.6 % / Mean I/σ(I) obs: 8 / Num. unique all: 2473 / Rsym value: 0.26 / % possible all: 99.4 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 65.913 Å2 / ksol: 0.336106 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 37.3 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2→32.65 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2→2.13 Å / Rfactor Rfree error: 0.026 / Total num. of bins used: 6
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Xplor file |
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