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- PDB-2zfl: Crystal Structure of the Kif1A Motor Domain during Mg release: Mg... -

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Basic information

Entry
Database: PDB / ID: 2zfl
TitleCrystal Structure of the Kif1A Motor Domain during Mg release: Mg-releasing Transition-3
ComponentsKinesin-like protein KIF1A, Kinesin heavy chain isoform 5C
KeywordsTRANSPORT PROTEIN / kinesin / Alpha and Beta Protein / Enzyme / ATPase / P-loop / Motor Protein / ATP-binding / Coiled coil / Microtubule / Nucleotide-binding
Function / homology
Function and homology information


anterograde dendritic transport of messenger ribonucleoprotein complex / distal axon / protein transport along microtubule / dendritic transport of messenger ribonucleoprotein complex / extrinsic component of neuronal dense core vesicle membrane / interkinetic nuclear migration / dense core granule cytoskeletal transport / anterograde neuronal dense core vesicle transport / Kinesins / anterograde dendritic transport of neurotransmitter receptor complex ...anterograde dendritic transport of messenger ribonucleoprotein complex / distal axon / protein transport along microtubule / dendritic transport of messenger ribonucleoprotein complex / extrinsic component of neuronal dense core vesicle membrane / interkinetic nuclear migration / dense core granule cytoskeletal transport / anterograde neuronal dense core vesicle transport / Kinesins / anterograde dendritic transport of neurotransmitter receptor complex / retrograde neuronal dense core vesicle transport / anterograde axonal protein transport / neuronal dense core vesicle / regulation of dendritic spine development / COPI-dependent Golgi-to-ER retrograde traffic / apolipoprotein receptor binding / cytoskeleton-dependent intracellular transport / regulation of dendritic spine morphogenesis / anterograde axonal transport / synaptic vesicle transport / motor neuron axon guidance / plus-end-directed microtubule motor activity / ciliary rootlet / kinesin complex / microtubule motor activity / microtubule-based movement / ATP metabolic process / mRNA transport / axon cytoplasm / axonal growth cone / dendrite cytoplasm / vesicle-mediated transport / presynapse / axon guidance / postsynapse / synaptic vesicle / microtubule binding / microtubule / axon / neuron projection / neuronal cell body / dendrite / ATP hydrolysis activity / perinuclear region of cytoplasm / protein-containing complex / ATP binding / identical protein binding / cytosol / cytoplasm
Similarity search - Function
Kinesin protein 1B / Kinesin-like KIF1-type / Kinesin-like / Kinesin protein / Kinesin-associated / Kinesin-associated / Kinesin motor domain / Kinesin / Kinesin-like protein / Forkhead-associated (FHA) domain profile. ...Kinesin protein 1B / Kinesin-like KIF1-type / Kinesin-like / Kinesin protein / Kinesin-associated / Kinesin-associated / Kinesin motor domain / Kinesin / Kinesin-like protein / Forkhead-associated (FHA) domain profile. / Forkhead associated domain / FHA domain / Forkhead-associated (FHA) domain / Kinesin motor domain signature. / Kinesin motor domain, conserved site / Kinesin motor, catalytic domain. ATPase. / Kinesin motor domain / Kinesin motor domain profile. / Kinesin motor domain / SMAD/FHA domain superfamily / Kinesin motor domain superfamily / PH domain / PH domain profile. / Pleckstrin homology domain. / Pleckstrin homology domain / PH-like domain superfamily / P-loop containing nucleoside triphosphate hydrolase / 3-Layer(aba) Sandwich / Alpha Beta
Similarity search - Domain/homology
ADENOSINE-5'-DIPHOSPHATE / Kinesin heavy chain isoform 5C / Kinesin-like protein KIF1A
Similarity search - Component
Biological speciesMus musculus (house mouse)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.7 Å
AuthorsNitta, R. / Okada, Y. / Hirokawa, N.
CitationJournal: Nat.Struct.Mol.Biol. / Year: 2008
Title: Structural model for strain-dependent microtubule activation of Mg-ADP release from kinesin.
Authors: Nitta, R. / Okada, Y. / Hirokawa, N.
History
DepositionJan 8, 2008Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Sep 23, 2008Provider: repository / Type: Initial release
Revision 1.1Jul 13, 2011Group: Version format compliance
Revision 1.2Aug 23, 2017Group: Source and taxonomy / Category: entity_src_gen

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Kinesin-like protein KIF1A, Kinesin heavy chain isoform 5C
hetero molecules


Theoretical massNumber of molelcules
Total (without water)41,5552
Polymers41,1281
Non-polymers4271
Water77543
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
Unit cell
Length a, b, c (Å)41.203, 51.857, 155.529
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121

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Components

#1: Protein Kinesin-like protein KIF1A, Kinesin heavy chain isoform 5C / Axonal transporter of synaptic vesi cles / Kinesin heavy chain neuron-specifiC 2


Mass: 41128.215 Da / Num. of mol.: 1 / Fragment: KIF1A (residues 1-355), KIF5C (residues 329-334)
Source method: isolated from a genetically manipulated source
Details: FUSION PROTEIN COMPRISES RESIDUES 1-355 OF Kinesin-like protein KIF1A, AND RESIDUES 329-334 OF Kinesin heavy chain isoform 5C, AND C-TERMINAL TAIL WITH SEQUENCE HHHHH
Source: (gene. exp.) Mus musculus (house mouse) / Plasmid: PET21B / Production host: Escherichia coli (E. coli) / References: UniProt: P33173, UniProt: P28738
#2: Chemical ChemComp-ADP / ADENOSINE-5'-DIPHOSPHATE / Adenosine diphosphate


Mass: 427.201 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C10H15N5O10P2 / Comment: ADP, energy-carrying molecule*YM
#3: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 43 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION

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Sample preparation

CrystalDensity Matthews: 2.02 Å3/Da / Density % sol: 39.11 %
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8.5
Details: pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K

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Data collection

DiffractionMean temperature: 93 K
Diffraction sourceSource: SYNCHROTRON / Site: Photon Factory / Beamline: AR-NW12A / Wavelength: 0.97 Å
DetectorType: ADSC QUANTUM 210 / Detector: CCD / Date: Mar 1, 2005
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97 Å / Relative weight: 1
ReflectionResolution: 2.7→50 Å / Num. obs: 8771 / Biso Wilson estimate: 21.4 Å2

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Processing

Software
NameVersionClassification
CNS1refinement
ADSCQuantumdata collection
HKL-2000data reduction
HKL-2000data scaling
CNSphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 1I5S
Resolution: 2.7→19.92 Å / Rfactor Rfree error: 0.011 / Data cutoff high absF: 209294.02 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0
RfactorNum. reflection% reflectionSelection details
Rfree0.325 868 10.7 %RANDOM
Rwork0.28 ---
obs0.28 8098 83.6 %-
Solvent computationSolvent model: FLAT MODEL / Bsol: 71.8349 Å2 / ksol: 0.330746 e/Å3
Displacement parametersBiso mean: 76.5 Å2
Baniso -1Baniso -2Baniso -3
1--9.83 Å20 Å20 Å2
2--51.56 Å20 Å2
3----41.73 Å2
Refine analyze
FreeObs
Luzzati coordinate error0.6 Å0.48 Å
Luzzati d res low-50 Å
Luzzati sigma a0.68 Å0.58 Å
Refinement stepCycle: LAST / Resolution: 2.7→19.92 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms2467 0 27 43 2537
Refine LS restraints
Refine-IDTypeDev idealDev ideal target
X-RAY DIFFRACTIONc_bond_d0.01
X-RAY DIFFRACTIONc_angle_deg1.3
X-RAY DIFFRACTIONc_dihedral_angle_d25.4
X-RAY DIFFRACTIONc_improper_angle_d0.9
X-RAY DIFFRACTIONc_mcbond_it3.721.5
X-RAY DIFFRACTIONc_mcangle_it6.042
X-RAY DIFFRACTIONc_scbond_it5.392
X-RAY DIFFRACTIONc_scangle_it7.592.5
LS refinement shellResolution: 2.7→2.87 Å / Rfactor Rfree error: 0.049 / Total num. of bins used: 6
RfactorNum. reflection% reflection
Rfree0.479 95 10.4 %
Rwork0.412 822 -
obs--57.7 %
Xplor file
Refine-IDSerial noParam fileTopol file
X-RAY DIFFRACTION1protein_rep.paramprotein.top
X-RAY DIFFRACTION2adp.paramadp.top
X-RAY DIFFRACTION3ion.paramion.top
X-RAY DIFFRACTION4water_rep.paramwater_rep.top

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