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- PDB-2z8c: Phosphorylated insulin receptor tyrosine kinase in complex with (... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2z8c | ||||||
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Title | Phosphorylated insulin receptor tyrosine kinase in complex with (4-{[5-carbamoyl-4-(3-methylanilino)pyrimidin-2-yl]amino}phenyl)acetic acid | ||||||
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![]() | TRANSFERASE / Alternative splicing / ATP-binding / Carbohydrate metabolism / Cleavage on pair of basic residues / Diabetes mellitus / Disease mutation / Glycoprotein / Kinase / Membrane / Nucleotide-binding / Phosphorylation / Polymorphism / Receptor / Transmembrane / Tyrosine-protein kinase / Transducer | ||||||
Function / homology | ![]() IRS-related events triggered by IGF1R / regulation of female gonad development / positive regulation of meiotic cell cycle / positive regulation of developmental growth / IRS-mediated signalling / positive regulation of fatty acid beta-oxidation / insulin-like growth factor II binding / male sex determination / exocrine pancreas development / insulin receptor complex ...IRS-related events triggered by IGF1R / regulation of female gonad development / positive regulation of meiotic cell cycle / positive regulation of developmental growth / IRS-mediated signalling / positive regulation of fatty acid beta-oxidation / insulin-like growth factor II binding / male sex determination / exocrine pancreas development / insulin receptor complex / positive regulation of glucose metabolic process / insulin-like growth factor I binding / insulin receptor activity / Activated NTRK3 signals through PI3K / transmembrane receptor protein tyrosine kinase adaptor activity / Signaling by Leptin / positive regulation of protein-containing complex disassembly / cargo receptor activity / PI3K/AKT activation / dendritic spine maintenance / cellular response to fatty acid / insulin binding / PTB domain binding / adrenal gland development / Signaling by ALK / neuronal cell body membrane / Signaling by Insulin receptor / IRS activation / activation of protein kinase activity / amyloid-beta clearance / positive regulation of respiratory burst / positive regulation of receptor internalization / regulation of embryonic development / transport across blood-brain barrier / SOS-mediated signalling / insulin receptor substrate binding / PI3K Cascade / positive regulation of glycogen biosynthetic process / epidermis development / negative regulation of insulin secretion / Signal attenuation / phosphatidylinositol 3-kinase binding / heart morphogenesis / Growth hormone receptor signaling / positive regulation of insulin receptor signaling pathway / dendrite membrane / Insulin receptor recycling / neuron projection maintenance / negative regulation of insulin receptor signaling pathway / Interleukin-7 signaling / positive regulation of glycolytic process / activation of protein kinase B activity / phosphotyrosine residue binding / SH2 domain binding / positive regulation of mitotic nuclear division / Insulin receptor signalling cascade / phosphatidylinositol 3-kinase/protein kinase B signal transduction / insulin-like growth factor receptor signaling pathway / receptor-mediated endocytosis / learning / caveola / positive regulation of glucose import / protein kinase C binding / insulin-like growth factor receptor binding / positive regulation of MAP kinase activity / insulin receptor binding / response to insulin / receptor internalization / receptor protein-tyrosine kinase / memory / cellular response to growth factor stimulus / cytokine-mediated signaling pathway / peptidyl-tyrosine phosphorylation / cellular response to insulin stimulus / Constitutive Signaling by Aberrant PI3K in Cancer / male gonad development / positive regulation of nitric oxide biosynthetic process / Signaling by ALK fusions and activated point mutants / signaling receptor complex adaptor activity / late endosome / insulin receptor signaling pathway / PIP3 activates AKT signaling / glucose homeostasis / amyloid-beta binding / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / RAF/MAP kinase cascade / protein tyrosine kinase activity / positive regulation of MAPK cascade / protein autophosphorylation / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / lysosome / receptor complex / endosome membrane / positive regulation of cell migration / symbiont entry into host cell / positive regulation of protein phosphorylation / G protein-coupled receptor signaling pathway / protein domain specific binding / external side of plasma membrane / axon Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Katayama, N. / Kurihara, H. | ||||||
![]() | ![]() Title: Identification of a key element for hydrogen-bonding patterns between protein kinases and their inhibitors. Authors: Katayama, N. / Orita, M. / Yamaguchi, T. / Hisamichi, H. / Kuromitsu, S. / Kurihara, H. / Sakashita, H. / Matsumoto, Y. / Fujita, S. / Niimi, T. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 76.1 KB | Display | ![]() |
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PDB format | ![]() | 56 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 739.2 KB | Display | ![]() |
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Full document | ![]() | 755.9 KB | Display | |
Data in XML | ![]() | 16.1 KB | Display | |
Data in CIF | ![]() | 20.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2z7lC ![]() 1ir3S C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 34689.320 Da / Num. of mol.: 1 / Fragment: TYROSINE KINASE DOMAIN / Mutation: C981S, Y984F Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: P06213, receptor protein-tyrosine kinase |
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#2: Protein/peptide | Mass: 759.849 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
#3: Chemical | ChemComp-S91 / [ |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.79 Å3/Da / Density % sol: 55.88 % |
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Crystal grow | Method: vapor diffusion, hanging drop / Details: PEG 3350, VAPOR DIFFUSION, HANGING DROP |
-Data collection
Diffraction source | Source: ![]() ![]() ![]() |
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Detector | Type: WEISSENBERG / Detector: DIFFRACTOMETER / Date: Jan 1, 2001 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 3.25→56.23 Å / Num. obs: 6395 / % possible obs: 98.7 % / Rmerge(I) obs: 0.092 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1IR3 Resolution: 3.25→56.23 Å / Rfactor Rfree error: 0.011 / Data cutoff high absF: 1313049.57 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 13.78 Å2 / ksol: 0.24 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 60.2 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 3.25→56.23 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.25→3.45 Å / Rfactor Rfree error: 0.036 / Total num. of bins used: 6
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