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Open data
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Basic information
Entry | Database: PDB / ID: 2z10 | ||||||
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Title | Crystal structure of putative acetyltransferase | ||||||
![]() | Ribosomal-protein-alanine acetyltransferase | ||||||
![]() | TRANSFERASE / alpha/beta protein / Acyltransferase / Structural Genomics / NPPSFA / National Project on Protein Structural and Functional Analyses / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | ![]() ribosomal-protein-alanine N-acetyltransferase / acyltransferase activity, transferring groups other than amino-acyl groups Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Murayama, K. / Kato-Murayama, M. / Terada, T. / Kuramitsu, S. / Shirouzu, M. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
![]() | ![]() Title: Genetic Encoding of 3-Iodo-l-Tyrosine in Escherichia coli for Single-Wavelength Anomalous Dispersion Phasing in Protein Crystallography Authors: Sakamoto, K. / Murayama, K. / Oki, K. / Iraha, F. / Kato-Murayama, M. / Takahashi, M. / Ohtake, K. / Kobayashi, T. / Kuramitsu, S. / Shirouzu, M. / Yokoyama, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 50.7 KB | Display | ![]() |
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PDB format | ![]() | 39.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 414.5 KB | Display | ![]() |
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Full document | ![]() | 414.9 KB | Display | |
Data in XML | ![]() | 10.9 KB | Display | |
Data in CIF | ![]() | 15.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 22288.467 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: Q72HN8, ribosomal-protein-alanine N-acetyltransferase |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.28 Å3/Da / Density % sol: 62.46 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 5.5 Details: 0.15M ammonium acetate, 0.1M bis-tris pH5.5, 28% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IV / Detector: IMAGE PLATE / Date: Jul 14, 2005 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54 Å / Relative weight: 1 |
Reflection | Resolution: 1.77→50 Å / Num. obs: 28933 / % possible obs: 98.9 % / Observed criterion σ(I): -3 / Redundancy: 9.7 % / Biso Wilson estimate: 25 Å2 / Rsym value: 0.042 / Net I/σ(I): 34.3 |
Reflection shell | Resolution: 1.77→1.83 Å / Redundancy: 9.3 % / Mean I/σ(I) obs: 8.9 / Rsym value: 0.266 / % possible all: 90.8 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 48.5752 Å2 / ksol: 0.348736 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 30 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.77→30.92 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.77→1.88 Å / Rfactor Rfree error: 0.021 / Total num. of bins used: 6
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Xplor file |
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