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- PDB-2yjt: Crystal structure of E. coli DEAD-box protein SrmB bound to regul... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2yjt | ||||||
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Title | Crystal structure of E. coli DEAD-box protein SrmB bound to regulator of ribonuclease activity A (RraA) | ||||||
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![]() | HYDROLASE INHIBITOR/HYDROLASE / HYDROLASE INHIBITOR-HYDROLASE COMPLEX / DEAD BOX RNA HELICASES | ||||||
Function / homology | ![]() negative regulation of RNA catabolic process / 4-hydroxy-4-methyl-2-oxoglutarate aldolase activity / ribonuclease inhibitor activity / oxaloacetate decarboxylase activity / RNA strand annealing activity / poly(A) binding / ATP-dependent activity, acting on RNA / protein homotrimerization / endoribonuclease inhibitor activity / ribosomal large subunit assembly ...negative regulation of RNA catabolic process / 4-hydroxy-4-methyl-2-oxoglutarate aldolase activity / ribonuclease inhibitor activity / oxaloacetate decarboxylase activity / RNA strand annealing activity / poly(A) binding / ATP-dependent activity, acting on RNA / protein homotrimerization / endoribonuclease inhibitor activity / ribosomal large subunit assembly / RNA helicase activity / RNA helicase / enzyme binding / ATP hydrolysis activity / protein-containing complex / ATP binding / identical protein binding / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Pietras, Z. / Hardwick, S.W. / Luisi, B.F. | ||||||
![]() | ![]() Title: Potential Regulatory Interactions of Escherichia Coli Rraa Protein with Dead-Box Helicases. Authors: Pietras, Z. / Hardwick, S.W. / Swiezewski, S. / Luisi, B.F. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 133.1 KB | Display | ![]() |
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PDB format | ![]() | 104.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 453.6 KB | Display | ![]() |
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Full document | ![]() | 474.5 KB | Display | |
Data in XML | ![]() | 26.3 KB | Display | |
Data in CIF | ![]() | 35.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2yjvC ![]() 1q5xS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Component-ID: 1 / Ens-ID: 1 / Beg auth comp-ID: LYS / Beg label comp-ID: LYS / End auth comp-ID: ASP / End label comp-ID: ASP / Refine code: 4 / Auth seq-ID: 2 - 159 / Label seq-ID: 2 - 159
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Components
#1: Protein | Mass: 17371.264 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Protein | | Mass: 19559.531 Da / Num. of mol.: 1 / Fragment: RESIDUES 219-388 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.47 Å3/Da / Density % sol: 50 % / Description: NONE |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, hanging drop / pH: 7.2 Details: CRYSTALS WERE PREPARED USING THE HANGING DROP METHOD AT 16 DEG. C BY MIXING 1:1 PROTEIN SAMPLE WITH MOTHER LIQUOR: 100 MM MAGNESIUM ACETATE, 100 MM MOPS PH 7.2 AND 12% W/V PEG 8000.THE ...Details: CRYSTALS WERE PREPARED USING THE HANGING DROP METHOD AT 16 DEG. C BY MIXING 1:1 PROTEIN SAMPLE WITH MOTHER LIQUOR: 100 MM MAGNESIUM ACETATE, 100 MM MOPS PH 7.2 AND 12% W/V PEG 8000.THE CRYSTALS WERE TRANSFERRED BRIEFLY TO RESERVOIR SOLUTION SUPPLEMENTED WITH 25% (V/V) GLYCEROL AND FLASH FROZEN IN LIQUID NITROGEN. |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD / Date: Jan 29, 2010 / Details: MIRRORS |
Radiation | Monochromator: SILICON CRYSTAL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9763 Å / Relative weight: 1 |
Reflection | Resolution: 2.9→63.56 Å / Num. obs: 15208 / % possible obs: 99.6 % / Observed criterion σ(I): 6 / Redundancy: 4 % / Rmerge(I) obs: 0.12 / Net I/σ(I): 10.2 |
Reflection shell | Resolution: 2.9→3.06 Å / Redundancy: 4.1 % / Rmerge(I) obs: 0.51 / Mean I/σ(I) obs: 2.8 / % possible all: 99.5 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1Q5X Resolution: 2.9→74.3 Å / Cor.coef. Fo:Fc: 0.928 / Cor.coef. Fo:Fc free: 0.882 / SU B: 16.027 / SU ML: 0.305 / Cross valid method: THROUGHOUT / ESU R Free: 0.435 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
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Displacement parameters | Biso mean: 39.431 Å2
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Refinement step | Cycle: LAST / Resolution: 2.9→74.3 Å
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Refine LS restraints |
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