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Yorodumi- PDB-2y9x: Crystal structure of PPO3, a tyrosinase from Agaricus bisporus, i... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2y9x | ||||||
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Title | Crystal structure of PPO3, a tyrosinase from Agaricus bisporus, in deoxy-form that contains additional unknown lectin-like subunit, with inhibitor tropolone | ||||||
Components |
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Keywords | OXIDOREDUCTASE / COPPER-CONTAINING / PIGMENTATION / TYPE-3 COPPER PROTEIN | ||||||
Function / homology | Function and homology information tyrosinase / tyrosinase activity / melanin biosynthetic process / metal ion binding Similarity search - Function | ||||||
Biological species | AGARICUS BISPORUS (button mushroom) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.78 Å | ||||||
Authors | Ismaya, W.T. / Rozeboom, H.J. / Weijn, A. / Mes, J.J. / Fusetti, F. / Wichers, H.J. / Dijkstra, B.W. | ||||||
Citation | Journal: Biochemistry / Year: 2011 Title: Crystal Structure of Agaricus Bisporus Mushroom Tyrosinase: Identity of the Tetramer Subunits and Interaction with Tropolone. Authors: Ismaya, W.T. / Rozeboom, H.J. / Weijn, A. / Mes, J.J. / Fusetti, F. / Wichers, H.J. / Dijkstra, B.W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2y9x.cif.gz | 832.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2y9x.ent.gz | 693.7 KB | Display | PDB format |
PDBx/mmJSON format | 2y9x.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2y9x_validation.pdf.gz | 517.6 KB | Display | wwPDB validaton report |
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Full document | 2y9x_full_validation.pdf.gz | 538.9 KB | Display | |
Data in XML | 2y9x_validation.xml.gz | 68.7 KB | Display | |
Data in CIF | 2y9x_validation.cif.gz | 92.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/y9/2y9x ftp://data.pdbj.org/pub/pdb/validation_reports/y9/2y9x | HTTPS FTP |
-Related structure data
Related structure data | 2y9wSC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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2 |
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4 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments:
NCS ensembles :
NCS oper:
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-Components
-Protein , 2 types, 8 molecules ABCDEFGH
#1: Protein | Mass: 45318.617 Da / Num. of mol.: 4 / Fragment: RESIDUES 2-392 / Source method: isolated from a natural source / Details: COMMERCIAL / Source: (natural) AGARICUS BISPORUS (button mushroom) / References: UniProt: C7FF04, tyrosinase #2: Protein | Mass: 16478.287 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Details: COMMERCIAL / Source: (natural) AGARICUS BISPORUS (button mushroom) / References: UniProt: G1K3P4*PLUS |
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-Non-polymers , 4 types, 41 molecules
#3: Chemical | ChemComp-CU / #4: Chemical | ChemComp-HO / #5: Chemical | ChemComp-0TR / #6: Water | ChemComp-HOH / | |
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-Details
Sequence details | NO DATABASE REFERENCE ORF 239342 OF A. BISPORUS GENOME CONSORTIUM |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.54 Å3/Da / Density % sol: 51.5 % / Description: NONE |
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Crystal grow | pH: 4.6 Details: 10% PEG 4000, 100 MM NA ACETATE PH 4.6, 5 MM HOLMIUM CHLORIDE. |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID23-2 / Wavelength: 0.8726 |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Nov 2, 2008 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.8726 Å / Relative weight: 1 |
Reflection | Resolution: 2.78→58 Å / Num. obs: 54004 / % possible obs: 89.6 % / Observed criterion σ(I): 0 / Redundancy: 2.7 % / Rmerge(I) obs: 0.24 / Net I/σ(I): 3.5 |
Reflection shell | Resolution: 2.78→2.93 Å / Redundancy: 2.6 % / Rmerge(I) obs: 0.62 / Mean I/σ(I) obs: 1.6 / % possible all: 89.7 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 2Y9W Resolution: 2.78→58.03 Å / Cor.coef. Fo:Fc: 0.87 / Cor.coef. Fo:Fc free: 0.802 / SU B: 42.963 / SU ML: 0.398 / Cross valid method: THROUGHOUT / ESU R Free: 0.491 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES WITH TLS ADDED.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 52.7 Å2
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Refinement step | Cycle: LAST / Resolution: 2.78→58.03 Å
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Refine LS restraints |
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