RNA Polymerase III Chain Elongation / RNA Polymerase III Transcription Termination / regulation of transcription by RNA polymerase III / RNA Polymerase III Transcription Initiation From Type 1 Promoter / RNA Polymerase III Transcription Initiation From Type 2 Promoter / RNA Polymerase III Transcription Initiation From Type 3 Promoter / Cytosolic sensors of pathogen-associated DNA / RNA Polymerase III Abortive And Retractive Initiation / positive regulation of innate immune response / RNA polymerase III complex ...RNA Polymerase III Chain Elongation / RNA Polymerase III Transcription Termination / regulation of transcription by RNA polymerase III / RNA Polymerase III Transcription Initiation From Type 1 Promoter / RNA Polymerase III Transcription Initiation From Type 2 Promoter / RNA Polymerase III Transcription Initiation From Type 3 Promoter / Cytosolic sensors of pathogen-associated DNA / RNA Polymerase III Abortive And Retractive Initiation / positive regulation of innate immune response / RNA polymerase III complex / positive regulation of interferon-beta production / DNA-directed RNA polymerase activity / single-stranded DNA binding / defense response to virus / innate immune response / DNA-templated transcription / nucleoplasm / cytosol 類似検索 - 分子機能
Helix Hairpins - #1450 / RNA polymerase III Rpc82, C -terminal / DNA-directed RNA polymerase III subunit RPC3 / : / RNA polymerase III subunit RPC82 / DNA-directed RNA polymerase III subunit RPC3, helical hairpin domain / POLR3C, C-terminal winged-helix domain / RNA polymerase III subunit RPC82-related, helix-turn-helix / RNA polymerase III subunit RPC82 helix-turn-helix domain / Helix Hairpins ...Helix Hairpins - #1450 / RNA polymerase III Rpc82, C -terminal / DNA-directed RNA polymerase III subunit RPC3 / : / RNA polymerase III subunit RPC82 / DNA-directed RNA polymerase III subunit RPC3, helical hairpin domain / POLR3C, C-terminal winged-helix domain / RNA polymerase III subunit RPC82-related, helix-turn-helix / RNA polymerase III subunit RPC82 helix-turn-helix domain / Helix Hairpins / Helix non-globular / Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain / Special / Arc Repressor Mutant, subunit A / Winged helix-like DNA-binding domain superfamily / Orthogonal Bundle / Mainly Alpha 類似検索 - ドメイン・相同性
DNA-directed RNA polymerase III subunit RPC3 類似検索 - 構成要素
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 0.98 Å / 相対比: 1
反射
解像度: 2.8→45 Å / Num. obs: 19023 / % possible obs: 99.3 % / Observed criterion σ(I): 2 / 冗長度: 5.26 % / Biso Wilson estimate: 74.74 Å2 / Rmerge(I) obs: 0.07 / Net I/σ(I): 14.1
反射 シェル
解像度: 2.8→2.9 Å / 冗長度: 5.19 % / Rmerge(I) obs: 0.62 / Mean I/σ(I) obs: 2 / % possible all: 98.2
-
解析
ソフトウェア
名称
バージョン
分類
BUSTER
2.9.1
精密化
XDS
データ削減
SCALEPACK
データスケーリング
SHELXD
位相決定
精密化
構造決定の手法: 単波長異常分散 開始モデル: NONE 解像度: 2.8→43.13 Å / SU R Cruickshank DPI: 0.428 / 交差検証法: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.398 / SU Rfree Blow DPI: 0.265 / SU Rfree Cruickshank DPI: 0.274
Rfactor
反射数
%反射
Selection details
Rfree
0.2298
943
4.96 %
RANDOM
Rwork
0.1884
-
-
-
obs
0.1905
18071
99.52 %
-
原子変位パラメータ
Biso mean: 101.77 Å2
Baniso -1
Baniso -2
Baniso -3
1-
15.2132 Å2
0 Å2
-6.6746 Å2
2-
-
2.4813 Å2
0 Å2
3-
-
-
-17.6945 Å2
Refine analyze
Luzzati coordinate error obs: 0.553 Å
精密化ステップ
サイクル: LAST / 解像度: 2.8→43.13 Å
タンパク質
核酸
リガンド
溶媒
全体
原子数
3436
0
0
13
3449
拘束条件
Refine-ID
タイプ
Dev ideal
数
Restraint function
Weight
X-RAY DIFFRACTION
t_bond_d
0.01
3480
HARMONIC
2
X-RAY DIFFRACTION
t_angle_deg
1.11
4690
HARMONIC
2
X-RAY DIFFRACTION
t_dihedral_angle_d
1277
SINUSOIDAL
2
X-RAY DIFFRACTION
t_incorr_chiral_ct
X-RAY DIFFRACTION
t_pseud_angle
X-RAY DIFFRACTION
t_trig_c_planes
94
HARMONIC
2
X-RAY DIFFRACTION
t_gen_planes
494
HARMONIC
5
X-RAY DIFFRACTION
t_it
3480
HARMONIC
20
X-RAY DIFFRACTION
t_nbd
X-RAY DIFFRACTION
t_omega_torsion
2.25
X-RAY DIFFRACTION
t_other_torsion
20.91
X-RAY DIFFRACTION
t_improper_torsion
X-RAY DIFFRACTION
t_chiral_improper_torsion
470
SEMIHARMONIC
5
X-RAY DIFFRACTION
t_sum_occupancies
X-RAY DIFFRACTION
t_utility_distance
X-RAY DIFFRACTION
t_utility_angle
X-RAY DIFFRACTION
t_utility_torsion
X-RAY DIFFRACTION
t_ideal_dist_contact
4036
SEMIHARMONIC
4
LS精密化 シェル
解像度: 2.8→2.95 Å / Total num. of bins used: 10
Rfactor
反射数
%反射
Rfree
0.2632
132
4.86 %
Rwork
0.2154
2586
-
all
0.2177
2718
-
obs
-
-
99.52 %
精密化 TLS
手法: refined / Origin x: 47.0892 Å / Origin y: -1.4398 Å / Origin z: 30.3491 Å