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Open data
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Basic information
Entry | Database: PDB / ID: 2xu9 | ||||||
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Title | Crystal structure of Laccase from Thermus thermophilus HB27 | ||||||
![]() | LACCASE | ||||||
![]() | OXIDOREDUCTASE / MULTICOPPER OXIDASES | ||||||
Function / homology | ![]() hydroquinone:oxygen oxidoreductase activity / laccase / ferroxidase activity / outer membrane-bounded periplasmic space / copper ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Serrano-Posada, H. / Valderrama, B. / Rudino-Pinera, E. | ||||||
![]() | ![]() Title: X-Ray-Induced Catalytic Active-Site Reduction of a Multicopper Oxidase: Structural Insights Into the Proton-Relay Mechanism and O2-Reduction States. Authors: Serrano-Posada, H. / Centeno-Leija, S. / Rojas-Trejo, S.P. / Rodriguez-Almazan, C. / Stojanoff, V. / Rudino-Pinera, E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 137.2 KB | Display | ![]() |
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PDB format | ![]() | 107.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 476.8 KB | Display | ![]() |
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Full document | ![]() | 500.3 KB | Display | |
Data in XML | ![]() | 29.2 KB | Display | |
Data in CIF | ![]() | 44.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2xuwC ![]() 2xvbC ![]() 2yaeC ![]() 2yafC ![]() 2yahC ![]() 2yamC ![]() 2yaoC ![]() 2yapC ![]() 2yaqC ![]() 2yarC ![]() 4ai7C ![]() 1kv7S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
-Protein , 1 types, 1 molecules A
#1: Protein | Mass: 48791.457 Da / Num. of mol.: 1 / Fragment: MATURE FORM, RESIDUES 24-462 / Mutation: YES Source method: isolated from a genetically manipulated source Details: THE PRESENCE OF AN ISOLEUCINE AT THE POSITION 53 IS STRONGLY SUPPORTED BY THE ELECTRON DENSITY. Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Non-polymers , 5 types, 570 molecules ![](data/chem/img/CU.gif)
![](data/chem/img/MPD.gif)
![](data/chem/img/MRD.gif)
![](data/chem/img/OH.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/MPD.gif)
![](data/chem/img/MRD.gif)
![](data/chem/img/OH.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | ChemComp-CU / #3: Chemical | ChemComp-MPD / ( #4: Chemical | ChemComp-MRD / ( #5: Chemical | ChemComp-OH / | #6: Water | ChemComp-HOH / | |
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-Details
Sequence details | THE SEQUENCE AT THE UNIPROT DEPOSIT Q4H436 POSITION 53 IS OCCUPIED BY A LEUCINE BUT THE ELECTRON ...THE SEQUENCE AT THE UNIPROT DEPOSIT Q4H436 POSITION 53 IS OCCUPIED BY A LEUCINE BUT THE ELECTRON DENSITY CLEARLY SUPPORTS THE PRESENCE OF AN ISOLEUCINE |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.6 Å3/Da / Density % sol: 52.7 % Description: PHASES WERE OBTAINED BY THE COMBINATION OF SIGNALS GENERATED BY MR AND SAD AT THE COOPER EDGE |
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Crystal grow | pH: 7.5 / Details: 0.1 M HEPES PH 7.5, 70% MPD |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD / Date: Nov 18, 2009 Details: DOUBLE CRYSTAL CHANNEL CUT, SI(111), 1M LONG RH COATED TOROIDAL MIRROR FOR VERTICAL AND HORIZONTAL FOCUSING. |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→23 Å / Num. obs: 78986 / % possible obs: 94.7 % / Observed criterion σ(I): 0 / Redundancy: 11.4 % / Biso Wilson estimate: 12.48 Å2 / Rmerge(I) obs: 0.09 / Net I/σ(I): 19.41 |
Reflection shell | Resolution: 1.5→1.6 Å / Redundancy: 9.9 % / Rmerge(I) obs: 0.4 / Mean I/σ(I) obs: 4.12 / % possible all: 83.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1KV7 Resolution: 1.501→22.816 Å / SU ML: 0.19 / σ(F): 1.39 / Phase error: 15.78 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 54.16 Å2 / ksol: 0.365 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 16.22 Å2
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Refinement step | Cycle: LAST / Resolution: 1.501→22.816 Å
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Refine LS restraints |
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LS refinement shell |
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