HELIX DETERMINATION METHOD: AUTHOR PROVIDED. SECONDARY STRUCTURE ASSIGNED BY DSSP DSSP OUTPUT ... HELIX DETERMINATION METHOD: AUTHOR PROVIDED. SECONDARY STRUCTURE ASSIGNED BY DSSP DSSP OUTPUT CONVERTED BY DSSP2PDB VERSION 0.03
Remark 700
SHEET DETERMINATION METHOD: AUTHOR PROVIDED. THE FOLLOWING SHEET RECORDS FOR MODEL `1` CHAIN ID ... SHEET DETERMINATION METHOD: AUTHOR PROVIDED. THE FOLLOWING SHEET RECORDS FOR MODEL `1` CHAIN ID `A` HAVE BEEN DETERMINED BY BETA-SPIDER, VERSION ALPHA 2.0 WITH AN ENERGY THRESHOLD OF -8.2 KCAL/MOL USING COULOMB ELECTROSTATICS USING 12-6 L-J VAN DER WAALS USING BETA-SPIDER RULE SETS.
LAMINARIHEPTAOSE, A BETA-1-3-LINKED GLUCAN FROM LAMINARIA DIGITATA, HAS BEEN ASSIGNED CHAIN B.
配列の詳細
POINT MUTATION OF NUCLEOPHILE RESIDUE GLUTAMATE 115 TO SERINE (THIS IS A E115S MUTANT)
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 1
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試料調製
結晶
マシュー密度: 2 Å3/Da / 溶媒含有率: 40 % 解説: RIGID BODY REFINEMENT WITH LAM16A APO STRUCTURE PDB 2CL2 AS MODEL
結晶化
温度: 293 K / pH: 5 詳細: 8 MG/ML PROTEIN SOLUTION IN 10 MM NAOAC WAS MIXED WITH AN EQUAL VOLUME OF CRYSTALLIZATION BUFFER CONTAINING 20% PEG 3350, 0.2 M AMMONIUM NITRATE AND 10 MM SODIUM ACETATE BUFFER, PH 5.0, AT 20 ...詳細: 8 MG/ML PROTEIN SOLUTION IN 10 MM NAOAC WAS MIXED WITH AN EQUAL VOLUME OF CRYSTALLIZATION BUFFER CONTAINING 20% PEG 3350, 0.2 M AMMONIUM NITRATE AND 10 MM SODIUM ACETATE BUFFER, PH 5.0, AT 20 DEGREES. SOAKING: 24 H IN 5 MM LAMINAROHEPTASACCHARIDE. CRYOPROTECTANT CONTAINED 35 % (W/V) PEG 3350, 0.2 M AMMONIUM NITRATE AND 10 MM SODIUM ACETATE BUFFER, PH 5.0
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データ収集
回折
平均測定温度: 100 K
放射光源
由来: シンクロトロン / サイト: MAX II / ビームライン: I911-3 / 波長: 0.983