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TitleSynthesis of Cyclic Beta-Glucan Using Laminarinase 16A Glycosynthase Mutant from the Basidiomycete Phanerochaete Chrysosporium.
Journal, issue, pagesJ. Am. Chem. Soc., Vol. 132, Page 1724-, Year 2010
Publish dateJun 24, 2009 (structure data deposition date)
AuthorsVasur, J. / Kawai, R. / Jonsson, K.H. / Widmalm, G. / Engstrom, A. / Frank, M. / Andersson, E. / Hansson, H. / Forsberg, Z. / Igarashi, K. ...Vasur, J. / Kawai, R. / Jonsson, K.H. / Widmalm, G. / Engstrom, A. / Frank, M. / Andersson, E. / Hansson, H. / Forsberg, Z. / Igarashi, K. / Samejima, M. / Sandgren, M. / Stahlberg, J.
External linksJ. Am. Chem. Soc. / PubMed:20078120
MethodsX-ray diffraction
Resolution1.4 - 2.124 Å
Structure data

PDB-2wlq:
Nucleophile-disabled Lam16A mutant holds laminariheptaose (L7) in a cyclical conformation
Method: X-RAY DIFFRACTION / Resolution: 1.4 Å

PDB-2wne:
Mutant Laminarinase 16A cyclizes laminariheptaose
Method: X-RAY DIFFRACTION / Resolution: 2.124 Å

Chemicals

ChemComp-HOH:
WATER

Source
  • phanerochaete chrysosporium (fungus)
KeywordsHYDROLASE / LAMINARIN / FAMILY 16 / CYCLICAL POYSACCHARIDES / GLYCOSYL HYDROLASE / BETA SANDWICH / BASIDIOMYCETE / BETA-GLUCANASE / GH7 / GH16 / LAM16A / BETA-1\ / 6-GLUCAN

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