+Open data
-Basic information
Entry | Database: PDB / ID: 2w1t | ||||||
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Title | Crystal Structure of B. subtilis SpoVT | ||||||
Components | (STAGE V SPORULATION PROTEIN T) x 2 | ||||||
Keywords | TRANSCRIPTION / TRANSCRIPTION REGULATION / REPRESSOR / ACTIVATOR / SPORULATION / DNA-BINDING / GAF DOMAIN REGULATED TETRAMERIC SPOVT | ||||||
Function / homology | Function and homology information sporulation resulting in formation of a cellular spore / DNA binding / identical protein binding Similarity search - Function | ||||||
Biological species | BACILLUS SUBTILIS (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.6 Å | ||||||
Authors | Asen, I. / Djuranovic, S. / Lupas, A.N. / Zeth, K. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2009 Title: Crystal Structure of Spovt, the Final Modulator of Gene Expression During Spore Development in Bacillus Subtilis Authors: Asen, I. / Djuranovic, S. / Lupas, A.N. / Zeth, K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2w1t.cif.gz | 141.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2w1t.ent.gz | 118.8 KB | Display | PDB format |
PDBx/mmJSON format | 2w1t.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/w1/2w1t ftp://data.pdbj.org/pub/pdb/validation_reports/w1/2w1t | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 19753.406 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) BACILLUS SUBTILIS (bacteria) / Strain: PY79 / Plasmid: PET30B / Production host: ESCHERICHIA COLI BL21(DE3) (bacteria) / Variant (production host): C41 / References: UniProt: P37554 |
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#2: Protein | Mass: 19767.434 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) BACILLUS SUBTILIS (bacteria) / Strain: PY79 / Plasmid: PET30B / Production host: ESCHERICHIA COLI BL21(DE3) (bacteria) / Variant (production host): C41 / References: UniProt: P37554 |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.2 Å3/Da / Density % sol: 61.1 % / Description: NONE |
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Crystal grow | pH: 6.5 Details: 1 M NACL, 100 MM CACODYLATE PH 6.5, 30% (V/V) PEG 600, 10% (V/V) GLYCEROL |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SLS / Beamline: X10SA / Wavelength: 0.978 |
Detector | Type: MARRESEARCH / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.978 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→20 Å / Num. obs: 18815 / % possible obs: 99.4 % / Observed criterion σ(I): 13.9 / Redundancy: 9.4 % / Rmerge(I) obs: 0.1 / Net I/σ(I): 13.9 |
Reflection shell | Resolution: 2.6→2.8 Å / Redundancy: 7.5 % / Rmerge(I) obs: 0.46 / Mean I/σ(I) obs: 3 / % possible all: 90.4 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.6→20 Å / Cross valid method: THROUGHOUT / σ(F): 2 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. RESIDUES AT THE N- AND C-TERMINUS ARE DISORDERED
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Refinement step | Cycle: LAST / Resolution: 2.6→20 Å
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