SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN ... SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN THE SHEET RECORDS BELOW, TWO SHEETS ARE DEFINED.
Mass: 18.015 Da / Num. of mol.: 198 / Source method: isolated from a natural source / Formula: H2O
Compound details
ENGINEERED RESIDUE IN CHAIN A, SER 1 TO ALA ENGINEERED RESIDUE IN CHAIN A, ILE 92 TO ALA ENGINEERED ...ENGINEERED RESIDUE IN CHAIN A, SER 1 TO ALA ENGINEERED RESIDUE IN CHAIN A, ILE 92 TO ALA ENGINEERED RESIDUE IN CHAIN B, SER 1 TO ALA ENGINEERED RESIDUE IN CHAIN B, ILE 92 TO ALA
Sequence details
RECOMBINANT FLD VARIANT LACKS THE FIRST METHIONINE AND THE SECOND AMINOACID IS AN ALANINE. ...RECOMBINANT FLD VARIANT LACKS THE FIRST METHIONINE AND THE SECOND AMINOACID IS AN ALANINE. ISOLEUCINE 92 IS MUTATED TO ALANINE
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.09 Å3/Da / Density % sol: 41.08 % / Description: NONE
Crystal grow
pH: 8.5 / Details: 30% PEG4K, 100 MM TRIS/HCL PH 8.5, 0.2 M MGCL2
Resolution: 1.99→26.13 Å / Cor.coef. Fo:Fc: 0.95 / Cor.coef. Fo:Fc free: 0.892 / SU B: 6.789 / SU ML: 0.182 / Cross valid method: THROUGHOUT / ESU R: 0.238 / ESU R Free: 0.217 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. IN CHAIN A, THERE IS NO ELECTRONIC DENSITY FOR THE FIRST TWO AMINOACIDS. IN CHAIN B, ALL AMINOACIDS ARE VISIBLE.
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.271
1056
5.2 %
RANDOM
Rwork
0.189
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obs
0.193
19386
94.8 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK