distal tubule morphogenesis / aldosterone secretion / chemokine (C-C motif) ligand 21 signaling pathway / renal sodium ion transport / regulation of potassium ion export across plasma membrane / negative regulation of pancreatic juice secretion / chemokine (C-X-C motif) ligand 12 signaling pathway / protein kinase C signaling / monoatomic ion homeostasis / negative regulation of sodium ion transport ...distal tubule morphogenesis / aldosterone secretion / chemokine (C-C motif) ligand 21 signaling pathway / renal sodium ion transport / regulation of potassium ion export across plasma membrane / negative regulation of pancreatic juice secretion / chemokine (C-X-C motif) ligand 12 signaling pathway / protein kinase C signaling / monoatomic ion homeostasis / negative regulation of sodium ion transport / negative regulation of potassium ion transmembrane transport / intracellular chloride ion homeostasis / renal sodium ion absorption / positive regulation of T cell chemotaxis / cellular hypotonic response / osmosensory signaling pathway / macrophage activation / cellular hyperosmotic response / cellular response to chemokine / cell volume homeostasis / negative regulation of protein localization to plasma membrane / chloride transport / chloride ion binding / ion channel inhibitor activity / response to dietary excess / sodium ion transmembrane transport / bicellular tight junction / calcium ion homeostasis / potassium ion transmembrane transport / ERK1 and ERK2 cascade / peptidyl-threonine phosphorylation / protein localization / Stimuli-sensing channels / regulation of blood pressure / cellular response to xenobiotic stimulus / cell body / gene expression / response to oxidative stress / protein autophosphorylation / non-specific serine/threonine protein kinase / protein kinase activity / intracellular signal transduction / response to xenobiotic stimulus / inflammatory response / protein phosphorylation / protein serine kinase activity / protein serine/threonine kinase activity / protein kinase binding / magnesium ion binding / signal transduction / protein-containing complex / extracellular exosome / ATP binding / membrane / identical protein binding / cytosol / cytoplasm Similarity search - Function
Serine/threonine-protein kinase OSR1/WNK, CCT domain / Oxidative-stress-responsive kinase 1 C-terminal domain / Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1 / Ubiquitin-like (UB roll) / Serine/threonine-protein kinase, active site / Serine/Threonine protein kinases active-site signature. / Protein kinase domain / Serine/Threonine protein kinases, catalytic domain / Roll / Protein kinase, ATP binding site ...Serine/threonine-protein kinase OSR1/WNK, CCT domain / Oxidative-stress-responsive kinase 1 C-terminal domain / Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1 / Ubiquitin-like (UB roll) / Serine/threonine-protein kinase, active site / Serine/Threonine protein kinases active-site signature. / Protein kinase domain / Serine/Threonine protein kinases, catalytic domain / Roll / Protein kinase, ATP binding site / Protein kinases ATP-binding region signature. / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily / Alpha Beta Similarity search - Domain/homology
SERINE/THREONINE-PROTEINKINASEOSR1 / OXIDATIVE STRESS-RESPONSIVE 1 PROTEIN
Mass: 10319.632 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) HOMO SAPIENS (human) / Production host: ESCHERICHIA COLI (E. coli) References: UniProt: O95747, non-specific serine/threonine protein kinase
#2: Protein/peptide
SERINE/THREONINE-PROTEINKINASEWNK4 / PROTEIN KINASE WITH NO LYSINE 4 / PROTEIN KINASE LYSINE-DEFICIENT 4
Mass: 707.798 Da / Num. of mol.: 2 / Fragment: RESIDUES 1015-1020 / Source method: obtained synthetically / Source: (synth.) HOMO SAPIENS (human) References: UniProt: Q96J92, non-specific serine/threonine protein kinase
Mass: 18.015 Da / Num. of mol.: 326 / Source method: isolated from a natural source / Formula: H2O
Compound details
REGULATES DOWNSTREAM KINASES IN RESPONSE TO ENVIRONMENTAL STRESS. MAY ALSO HAVE A FUNCTION IN ...REGULATES DOWNSTREAM KINASES IN RESPONSE TO ENVIRONMENTAL STRESS. MAY ALSO HAVE A FUNCTION IN REGULATING THE ACTIN CYTOSKELETON.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION
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Sample preparation
Crystal
Density Matthews: 1.8 Å3/Da / Density % sol: 33 % / Description: NONE
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Data collection
Diffraction
Mean temperature: 100 K
Diffraction source
Source: ROTATING ANODE / Wavelength: 1.54
Radiation
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 1.54 Å / Relative weight: 1
Reflection
Resolution: 1.7→20 Å / Num. obs: 20989 / % possible obs: 91 % / Observed criterion σ(I): 3 / Redundancy: 3.4 % / Rmerge(I) obs: 0.04 / Net I/σ(I): 33.1
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Processing
Software
Name
Version
Classification
REFMAC
5.2.0019
refinement
DENZO
datareduction
SCALEPACK
datascaling
MOLREP
phasing
Refinement
Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.7→20 Å / Cor.coef. Fo:Fc: 0.956 / Cor.coef. Fo:Fc free: 0.945 / SU B: 2.387 / SU ML: 0.082 / Cross valid method: THROUGHOUT / ESU R: 0.139 / ESU R Free: 0.131 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.239
634
3.2 %
RANDOM
Rwork
0.198
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obs
0.2
19179
94.8 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK