distal tubule morphogenesis / aldosterone secretion / chemokine (C-C motif) ligand 21 signaling pathway / renal sodium ion transport / regulation of potassium ion export across plasma membrane / negative regulation of pancreatic juice secretion / chemokine (C-X-C motif) ligand 12 signaling pathway / protein kinase C signaling / monoatomic ion homeostasis / negative regulation of sodium ion transport ...distal tubule morphogenesis / aldosterone secretion / chemokine (C-C motif) ligand 21 signaling pathway / renal sodium ion transport / regulation of potassium ion export across plasma membrane / negative regulation of pancreatic juice secretion / chemokine (C-X-C motif) ligand 12 signaling pathway / protein kinase C signaling / monoatomic ion homeostasis / negative regulation of sodium ion transport / negative regulation of potassium ion transmembrane transport / positive regulation of potassium ion import across plasma membrane / intracellular chloride ion homeostasis / renal sodium ion absorption / positive regulation of T cell chemotaxis / cellular hypotonic response / macrophage activation / potassium channel inhibitor activity / cellular response to chemokine / cellular hyperosmotic response / osmosensory signaling pathway / cell volume homeostasis / negative regulation of protein localization to plasma membrane / chloride transport / chloride ion binding / ion channel inhibitor activity / response to dietary excess / sodium ion transmembrane transport / bicellular tight junction / calcium ion homeostasis / potassium ion transmembrane transport / ERK1 and ERK2 cascade / peptidyl-threonine phosphorylation / protein localization / Stimuli-sensing channels / regulation of blood pressure / cellular response to xenobiotic stimulus / gene expression / cell body / response to oxidative stress / protein autophosphorylation / non-specific serine/threonine protein kinase / protein kinase activity / intracellular signal transduction / response to xenobiotic stimulus / inflammatory response / protein phosphorylation / protein serine kinase activity / protein serine/threonine kinase activity / protein kinase binding / magnesium ion binding / signal transduction / protein-containing complex / extracellular exosome / ATP binding / identical protein binding / membrane / cytoplasm / cytosol 類似検索 - 分子機能
Serine/threonine-protein kinase OSR1/WNK, CCT domain / Oxidative-stress-responsive kinase 1 C-terminal domain / Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1 / Ubiquitin-like (UB roll) / Serine/threonine-protein kinase, active site / Serine/Threonine protein kinases active-site signature. / Protein kinase domain / Serine/Threonine protein kinases, catalytic domain / Roll / Protein kinase, ATP binding site ...Serine/threonine-protein kinase OSR1/WNK, CCT domain / Oxidative-stress-responsive kinase 1 C-terminal domain / Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1 / Ubiquitin-like (UB roll) / Serine/threonine-protein kinase, active site / Serine/Threonine protein kinases active-site signature. / Protein kinase domain / Serine/Threonine protein kinases, catalytic domain / Roll / Protein kinase, ATP binding site / Protein kinases ATP-binding region signature. / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily / Alpha Beta 類似検索 - ドメイン・相同性
REGULATES DOWNSTREAM KINASES IN RESPONSE TO ENVIRONMENTAL STRESS. MAY ALSO HAVE A FUNCTION IN ...REGULATES DOWNSTREAM KINASES IN RESPONSE TO ENVIRONMENTAL STRESS. MAY ALSO HAVE A FUNCTION IN REGULATING THE ACTIN CYTOSKELETON.
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実験情報
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実験
実験
手法: X線回折
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試料調製
結晶
マシュー密度: 1.8 Å3/Da / 溶媒含有率: 33 % / 解説: NONE
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データ収集
回折
平均測定温度: 100 K
放射光源
由来: 回転陽極 / 波長: 1.54
放射
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 1.54 Å / 相対比: 1
反射
解像度: 1.7→20 Å / Num. obs: 20989 / % possible obs: 91 % / Observed criterion σ(I): 3 / 冗長度: 3.4 % / Rmerge(I) obs: 0.04 / Net I/σ(I): 33.1
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解析
ソフトウェア
名称
バージョン
分類
REFMAC
5.2.0019
精密化
DENZO
データ削減
SCALEPACK
データスケーリング
MOLREP
位相決定
精密化
構造決定の手法: 分子置換 / 解像度: 1.7→20 Å / Cor.coef. Fo:Fc: 0.956 / Cor.coef. Fo:Fc free: 0.945 / SU B: 2.387 / SU ML: 0.082 / 交差検証法: THROUGHOUT / ESU R: 0.139 / ESU R Free: 0.131 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
Rfactor
反射数
%反射
Selection details
Rfree
0.239
634
3.2 %
RANDOM
Rwork
0.198
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obs
0.2
19179
94.8 %
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.4 Å / 溶媒モデル: MASK