+Open data
-Basic information
Entry | Database: PDB / ID: 2v1x | ||||||
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Title | Crystal structure of human RECQ-like DNA helicase | ||||||
Components | ATP-DEPENDENT DNA HELICASE Q1 | ||||||
Keywords | HYDROLASE / DNA STRAND ANNEALING / MISMATCH REPAIR / NUCLEOTIDE-BINDING / DNA-BINDING / NUCLEAR PROTEIN / ATPASE / HELICASE / ATP-BINDING | ||||||
Function / homology | Function and homology information double-stranded DNA helicase activity / DNA/DNA annealing activity / four-way junction helicase activity / mediator complex / DNA 3'-5' helicase / replication fork processing / DNA unwinding involved in DNA replication / 3'-5' DNA helicase activity / DNA helicase activity / isomerase activity ...double-stranded DNA helicase activity / DNA/DNA annealing activity / four-way junction helicase activity / mediator complex / DNA 3'-5' helicase / replication fork processing / DNA unwinding involved in DNA replication / 3'-5' DNA helicase activity / DNA helicase activity / isomerase activity / double-strand break repair via homologous recombination / chromosome / DNA repair / ATP hydrolysis activity / nucleoplasm / ATP binding / membrane / nucleus / metal ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | HOMO SAPIENS (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2 Å | ||||||
Authors | Pike, A.C.W. / Shrestha, B. / Burgess-Brown, N. / King, O. / Ugochukwu, E. / Watt, S. / Edwards, A. / Arrowsmith, C.H. / Weigelt, J. / Sundstrom, M. / Gileadi, O. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2009 Title: Structure of the Human Recq1 Helicase Reveals a Putative Strand-Separation Pin. Authors: Pike, A.C.W. / Shrestha, B. / Popuri, V. / Burgess-Brown, N. / Muzzolini, L. / Costantini, S. / Vindigni, A. / Gileadi, O. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2v1x.cif.gz | 230.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2v1x.ent.gz | 179.8 KB | Display | PDB format |
PDBx/mmJSON format | 2v1x.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2v1x_validation.pdf.gz | 1.1 MB | Display | wwPDB validaton report |
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Full document | 2v1x_full_validation.pdf.gz | 1.1 MB | Display | |
Data in XML | 2v1x_validation.xml.gz | 42 KB | Display | |
Data in CIF | 2v1x_validation.cif.gz | 60 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/v1/2v1x ftp://data.pdbj.org/pub/pdb/validation_reports/v1/2v1x | HTTPS FTP |
-Related structure data
Related structure data | 1oyyS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments:
NCS oper: (Code: given Matrix: (1, 0.017, -0.001), Vector: |
-Components
-Protein , 1 types, 2 molecules AB
#1: Protein | Mass: 67384.938 Da / Num. of mol.: 2 / Fragment: RESIDUES 49-616 Source method: isolated from a genetically manipulated source Source: (gene. exp.) HOMO SAPIENS (human) / Plasmid: PNIC-CTHF / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / Variant (production host): R3-PRARE2 References: UniProt: P46063, Hydrolases; Acting on acid anhydrides; In phosphorus-containing anhydrides |
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-Non-polymers , 6 types, 332 molecules
#2: Chemical | #3: Chemical | #4: Chemical | #5: Chemical | ChemComp-CL / #6: Chemical | #7: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.51 Å3/Da / Density % sol: 51.01 % |
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Crystal grow | pH: 7.5 Details: 0.2M SODIUM BROMIDE,20% PEG3350, 10% ETHYLENE GLYCOL, 0.1M BIS TRIS PROPANE PH7.5, pH 7.50 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SLS / Beamline: X10SA / Wavelength: 1.03315 |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Apr 16, 2007 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.03315 Å / Relative weight: 1 |
Reflection | Resolution: 2→45.74 Å / Num. obs: 89784 / % possible obs: 100 % / Observed criterion σ(I): 0 / Redundancy: 3.7 % / Biso Wilson estimate: 31.96 Å2 / Rmerge(I) obs: 0.07 / Net I/σ(I): 10.4 |
Reflection shell | Resolution: 2→2.11 Å / Redundancy: 3.2 % / Rmerge(I) obs: 0.62 / Mean I/σ(I) obs: 1.6 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1OYY Resolution: 2→45 Å / Cor.coef. Fo:Fc: 0.953 / Cor.coef. Fo:Fc free: 0.931 / SU B: 6.493 / SU ML: 0.174 / Cross valid method: THROUGHOUT / ESU R: 0.195 / ESU R Free: 0.18 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 33.88 Å2
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Refinement step | Cycle: LAST / Resolution: 2→45 Å
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Refine LS restraints |
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