Pseudoazurin with engineered amicyanin ligand loop, reduced form, pH 7.5
要素
PSEUDOAZURIN
キーワード
ELECTRON TRANSPORT / TYPE-1 COPPER / METAL-BINDING / REDOX POTENTIAL / COPPER / TRANSPORT / CUPREDOXIN / PERIPLASMIC / SPECTROSCOPIC PROPERTIES / LOOP SHORTENING / PROTEIN SCAFFOLD / ELECTRON TRANSFER
機能・相同性
機能・相同性情報
electron transporter, transferring electrons from cytochrome b6/f complex of photosystem II activity / periplasmic space / copper ion binding 類似検索 - 分子機能
Pseudoazurin / Amicyanin/Pseudoazurin / Blue (type 1) copper protein, plastocyanin-type / Blue (type 1) copper domain / Copper binding proteins, plastocyanin/azurin family / Blue (type 1) copper protein, binding site / Type-1 copper (blue) proteins signature. / Cupredoxins - blue copper proteins / Cupredoxin / Immunoglobulin-like ...Pseudoazurin / Amicyanin/Pseudoazurin / Blue (type 1) copper protein, plastocyanin-type / Blue (type 1) copper domain / Copper binding proteins, plastocyanin/azurin family / Blue (type 1) copper protein, binding site / Type-1 copper (blue) proteins signature. / Cupredoxins - blue copper proteins / Cupredoxin / Immunoglobulin-like / Sandwich / Mainly Beta 類似検索 - ドメイン・相同性
THE SEQUENCE OF THE MATURE PROTEIN PRESENT IN THE CRYSTALS STARTS ALA29 AND HAS 125 RESIDUES. ...THE SEQUENCE OF THE MATURE PROTEIN PRESENT IN THE CRYSTALS STARTS ALA29 AND HAS 125 RESIDUES. RESIDUES BETWEEN 109 AND 114 WERE DELETED AND REPLACED BY PRO PHE
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 1
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試料調製
結晶
マシュー密度: 2.12 Å3/Da / 溶媒含有率: 41.61 % / 解説: THE CRYSTALS WERE REDUCED WITH MERCAPTOETHANOL
結晶化
手法: 蒸気拡散法, ハンギングドロップ法 / pH: 7.5 詳細: HANGING DROP VAPOR DIFFUSION METHOD, 1 MICRO-L OF PROTEIN (20MG/ML IN 50 MM TRIS/HCL PLUS 30 MM NACL) WERE MIXED WITH 1 MICRO-L RESORVOIR SOLUTION (10 MM TRIS/HCL PLUS 2 M AMMONIUM SULFATE ...詳細: HANGING DROP VAPOR DIFFUSION METHOD, 1 MICRO-L OF PROTEIN (20MG/ML IN 50 MM TRIS/HCL PLUS 30 MM NACL) WERE MIXED WITH 1 MICRO-L RESORVOIR SOLUTION (10 MM TRIS/HCL PLUS 2 M AMMONIUM SULFATE AND 2 M NACL). THE CRYSTALS WERE REDUCED BY ADDING 50 MM MERCAPTOETHANOL TO THE RESERVOIR SOLUTION AND BECAME COLORLESS AFTER 3 HOURS., pH 7.5
解像度: 1.3→30 Å / Cor.coef. Fo:Fc: 0.963 / Cor.coef. Fo:Fc free: 0.961 / SU B: 1.473 / SU ML: 0.032 / 交差検証法: THROUGHOUT / ESU R: 0.053 / ESU R Free: 0.052 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. SOME OF THE SIDE CHAINS WERE MODELED WITH TWO ALTERNATE CONFORMATIONS. THE COPPER AND SULFUR ATOMS WERE REFINED ANISOTROPICALLY.
Rfactor
反射数
%反射
Selection details
Rfree
0.201
1503
5.1 %
RANDOM
Rwork
0.192
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obs
0.192
27910
97.6 %
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.4 Å / 溶媒モデル: BABINET MODEL WITH MASK