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Open data
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Basic information
Entry | Database: PDB / ID: 2tn4 | ||||||
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Title | FOUR CALCIUM TNC | ||||||
![]() | TROPONIN C | ||||||
![]() | CONTRACTILE SYSTEM PROTEIN / CALCIUM REGULATION / CALMODULIN SUPERFAMILY | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Love, M.L. / Dominguez, R. / Houdusse, A. / Cohen, C. | ||||||
![]() | ![]() Title: Structures of four Ca2+-bound troponin C at 2.0 A resolution: further insights into the Ca2+-switch in the calmodulin superfamily. Authors: Houdusse, A. / Love, M.L. / Dominguez, R. / Grabarek, Z. / Cohen, C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 47.5 KB | Display | ![]() |
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PDB format | ![]() | 33 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 363.1 KB | Display | ![]() |
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Full document | ![]() | 364.8 KB | Display | |
Data in XML | ![]() | 4.8 KB | Display | |
Data in CIF | ![]() | 7.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1tn4SC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 17991.801 Da / Num. of mol.: 1 / Mutation: C98L Source method: isolated from a genetically manipulated source Details: RABBIT SKELETAL TROPONIN C / Source: (gene. exp.) ![]() ![]() ![]() ![]() | ||
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#2: Chemical | ChemComp-CA / #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.6 Å3/Da / Density % sol: 53 % | ||||||||||||||||||||||||
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Crystal grow | pH: 7.2 Details: 54% MPD 50 MM HEPES, PH 7.2 10 MM CACL2 1 MM NA-AZIDE | ||||||||||||||||||||||||
Crystal | *PLUS | ||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / Method: vapor diffusion / Details: used to seeding | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 160 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM / Detector: CCD / Date: Mar 2, 1997 |
Radiation | Monochromator: 0.9 / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.91 Å / Relative weight: 1 |
Reflection | Resolution: 2→20 Å / Num. obs: 12852 / % possible obs: 94.4 % / Redundancy: 10.1 % / Rmerge(I) obs: 0.109 / Rsym value: 0.109 / Net I/σ(I): 25 |
Reflection shell | Resolution: 2→2.7 Å / Redundancy: 2.8 % / Rmerge(I) obs: 0.185 / Mean I/σ(I) obs: 27 / Rsym value: 0.185 / % possible all: 93.1 |
Reflection | *PLUS Num. measured all: 168056 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1TN4 Resolution: 2→10 Å / Cross valid method: A POSTERIORI / Details: X-PLOR (BRUNGER) ALSO WAS USED.
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Refinement step | Cycle: LAST / Resolution: 2→10 Å
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Refine LS restraints |
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Software | *PLUS Name: ARP / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.212 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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