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- PDB-2tgi: CRYSTAL STRUCTURE OF TRANSFORMING GROWTH FACTOR-BETA2: AN UNUSUAL... -

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Basic information

Entry
Database: PDB / ID: 2tgi
TitleCRYSTAL STRUCTURE OF TRANSFORMING GROWTH FACTOR-BETA2: AN UNUSUAL FOLD FOR THE SUPERFAMILY
ComponentsTRANSFORMING GROWTH FACTOR ,BETA 2
KeywordsGROWTH FACTOR
Function / homology
Function and homology information


regulation of timing of catagen / regulation of apoptotic process involved in outflow tract morphogenesis / negative regulation of epithelial to mesenchymal transition involved in endocardial cushion formation / substantia propria of cornea development / ascending aorta morphogenesis / positive regulation of activation-induced cell death of T cells / cardioblast differentiation / uterine wall breakdown / positive regulation of timing of catagen / positive regulation of cardioblast differentiation ...regulation of timing of catagen / regulation of apoptotic process involved in outflow tract morphogenesis / negative regulation of epithelial to mesenchymal transition involved in endocardial cushion formation / substantia propria of cornea development / ascending aorta morphogenesis / positive regulation of activation-induced cell death of T cells / cardioblast differentiation / uterine wall breakdown / positive regulation of timing of catagen / positive regulation of cardioblast differentiation / cardiac right ventricle morphogenesis / regulation of transforming growth factor beta2 production / atrial septum morphogenesis / pharyngeal arch artery morphogenesis / type III transforming growth factor beta receptor binding / positive regulation of epithelial to mesenchymal transition involved in endocardial cushion formation / positive regulation of heart contraction / activation-induced cell death of T cells / glial cell migration / positive regulation of extracellular matrix disassembly / secondary palate development / negative regulation of macrophage cytokine production / somatic stem cell division / positive regulation of integrin biosynthetic process / atrial septum primum morphogenesis / endocardial cushion fusion / membranous septum morphogenesis / heart valve morphogenesis / negative regulation of cartilage development / cardiac epithelial to mesenchymal transition / signaling / TGFBR3 regulates TGF-beta signaling / positive regulation of stress-activated MAPK cascade / neuron fate commitment / pericyte cell differentiation / transforming growth factor beta receptor binding / eye development / neural retina development / embryonic digestive tract development / type II transforming growth factor beta receptor binding / pulmonary valve morphogenesis / cranial skeletal system development / ventricular trabecula myocardium morphogenesis / negative regulation of Ras protein signal transduction / embryo development ending in birth or egg hatching / outflow tract septum morphogenesis / positive regulation of extrinsic apoptotic signaling pathway in absence of ligand / cell-cell junction organization / collagen fibril organization / embryonic limb morphogenesis / positive regulation of cell adhesion mediated by integrin / atrioventricular valve morphogenesis / face morphogenesis / odontogenesis / endocardial cushion morphogenesis / Molecules associated with elastic fibres / hair follicle morphogenesis / cartilage condensation / dopamine biosynthetic process / generation of neurons / ventricular septum morphogenesis / positive regulation of Notch signaling pathway / uterus development / inner ear development / TGF-beta receptor signaling activates SMADs / hemopoiesis / positive regulation of cell division / positive regulation of SMAD protein signal transduction / positive regulation of epithelial cell migration / epithelial to mesenchymal transition / blood vessel remodeling / hair follicle development / ECM proteoglycans / cardiac muscle cell proliferation / neuron development / positive regulation of epithelial to mesenchymal transition / salivary gland morphogenesis / heart morphogenesis / positive regulation of cell cycle / extrinsic apoptotic signaling pathway / neutrophil chemotaxis / extrinsic apoptotic signaling pathway in absence of ligand / epithelial cell differentiation / transforming growth factor beta receptor signaling pathway / axon guidance / response to progesterone / platelet alpha granule lumen / negative regulation of angiogenesis / cytokine activity / skeletal system development / positive regulation of protein secretion / neural tube closure / regulation of actin cytoskeleton organization / growth factor activity / kidney development / wound healing / negative regulation of cell growth / positive regulation of immune response / response to wounding / positive regulation of miRNA transcription
Similarity search - Function
Transforming growth factor beta-2 proprotein / Transforming growth factor-beta / TGF-beta, propeptide / TGF-beta propeptide / Transforming growth factor beta, conserved site / TGF-beta family signature. / Transforming growth factor-beta-related / Transforming growth factor-beta (TGF-beta) family / Cystine Knot Cytokines, subunit B / Cystine-knot cytokines ...Transforming growth factor beta-2 proprotein / Transforming growth factor-beta / TGF-beta, propeptide / TGF-beta propeptide / Transforming growth factor beta, conserved site / TGF-beta family signature. / Transforming growth factor-beta-related / Transforming growth factor-beta (TGF-beta) family / Cystine Knot Cytokines, subunit B / Cystine-knot cytokines / Transforming growth factor-beta, C-terminal / Transforming growth factor beta like domain / TGF-beta family profile. / Cystine-knot cytokine / Ribbon / Mainly Beta
Similarity search - Domain/homology
Transforming growth factor beta-2 proprotein
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / Resolution: 1.8 Å
AuthorsDaopin, S. / Davies, D.R.
Citation
Journal: Science / Year: 1992
Title: Crystal structure of transforming growth factor-beta 2: an unusual fold for the superfamily.
Authors: Daopin, S. / Piez, K.A. / Ogawa, Y. / Davies, D.R.
#1: Journal: Proteins / Year: 1993
Title: Crystal Structure of Tgf-Beta2 Refined at 1.8 Angstroms Resolution
Authors: Daopin, S. / Li, M. / Davies, D.R.
History
DepositionOct 20, 1993Processing site: BNL
Revision 1.0Jan 31, 1994Provider: repository / Type: Initial release
Revision 1.1Mar 25, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Derived calculations / Version format compliance
Revision 1.3Nov 29, 2017Group: Derived calculations / Other
Category: pdbx_database_status / struct_conf / struct_conf_type
Item: _pdbx_database_status.process_site
Revision 1.4Oct 16, 2024Group: Data collection / Database references / Structure summary
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_entry_details / pdbx_modification_feature
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: TRANSFORMING GROWTH FACTOR ,BETA 2


Theoretical massNumber of molelcules
Total (without water)12,7331
Polymers12,7331
Non-polymers00
Water1,04558
1
A: TRANSFORMING GROWTH FACTOR ,BETA 2

A: TRANSFORMING GROWTH FACTOR ,BETA 2


Theoretical massNumber of molelcules
Total (without water)25,4652
Polymers25,4652
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation6_556-x,-x+y,-z+5/31
Buried area2520 Å2
ΔGint-28 kcal/mol
Surface area11790 Å2
MethodPISA, PQS
Unit cell
Length a, b, c (Å)60.600, 60.600, 75.300
Angle α, β, γ (deg.)90.00, 90.00, 120.00
Int Tables number154
Space group name H-MP3221
Atom site foot note1: RESIDUE PRO 36 IS A CIS PROLINE.
2: RESIDUES 92 - 96 ARE ILL-DEFINED IN THE ELECTRON DENSITY. MAP.

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Components

#1: Protein TRANSFORMING GROWTH FACTOR ,BETA 2


Mass: 12732.597 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / References: UniProt: P61812
#2: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 58 / Source method: isolated from a natural source / Formula: H2O
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION

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Sample preparation

CrystalDensity Matthews: 3.13 Å3/Da / Density % sol: 60.74 %
Crystal grow
*PLUS
Method: vapor diffusion
Components of the solutions
*PLUS
IDConc.Common nameCrystal-IDSol-ID
110 mMacetate1drop
220 %PEG2001reservoir
350 mMsodium acetate1reservoir
430-50 mMunbuffered sodium acetate1reservoir

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Data collection

Reflection
*PLUS
Highest resolution: 2.1 Å / Num. obs: 9706 / % possible obs: 87 % / Num. measured all: 54248 / Rmerge(I) obs: 0.047

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Processing

SoftwareName: TNT / Classification: refinement
RefinementResolution: 1.8→8 Å / Rfactor obs: 0.173
Refinement stepCycle: LAST / Resolution: 1.8→8 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms890 0 0 58 948
Refine LS restraints
Refine-IDTypeDev ideal
X-RAY DIFFRACTIONt_bond_d0.017
X-RAY DIFFRACTIONt_angle_deg2.28
X-RAY DIFFRACTIONt_dihedral_angle_d
X-RAY DIFFRACTIONt_incorr_chiral_ct
X-RAY DIFFRACTIONt_pseud_angle
X-RAY DIFFRACTIONt_trig_c_planes
X-RAY DIFFRACTIONt_gen_planes
X-RAY DIFFRACTIONt_it
X-RAY DIFFRACTIONt_nbd
Software
*PLUS
Name: TNT / Classification: refinement
Refinement
*PLUS
Highest resolution: 2.1 Å / Lowest resolution: 15 Å / Rfactor obs: 0.172 / Rfactor Rwork: 0.172
Solvent computation
*PLUS
Displacement parameters
*PLUS
Refine LS restraints
*PLUS
Refine-IDTypeDev ideal
X-RAY DIFFRACTIONt_angle_d
X-RAY DIFFRACTIONt_angle_deg2.3
X-RAY DIFFRACTIONt_plane_restr
X-RAY DIFFRACTIONt_bond_d0.016
X-RAY DIFFRACTIONt_planar_d0.016

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